The diagnostic performance and accuracy of 3 molecular assays for the detection of Clostridium difficile in stool samples, compared with the Xpert® C. difficile assay
| Autor: | Maya Azrad, Linda Tkhawkho, Avi Peretz, Zohar Hamo |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Microbiology (medical)
0303 health sciences medicine.medical_specialty 030306 microbiology business.industry Clostridioides difficile Reproducibility of Results Diagnostic accuracy Clostridium difficile C difficile Microbiology Gastroenterology Sensitivity and Specificity 03 medical and health sciences Feces Molecular Diagnostic Techniques Internal medicine medicine Clostridium Infections Humans Reagent Kits Diagnostic business Molecular Biology Algorithms 030304 developmental biology |
| Zdroj: | Journal of microbiological methods. 168 |
| ISSN: | 1872-8359 |
| Popis: | This research compares the performance and diagnostic accuracy of three molecular tests for the detection of Clostridium difficile in stool samples, with the Xpert® C. difficile assay. Fifty-nine positive and twenty-five stool negative samples were analyzed by the BDmax™ Cdiff, the Simplexa C. difficile Direct, and the GenomEra™ C. difficile, and compared with the Xpert® C. difficile routinely used in our laboratory. The highest sensitivity was 94.9% for the BDmax™ Cdiff, followed by GenomEra™ C. difficile with 93.2%, and Simplexa™ C. difficile Direct with 89.8%. The specificities of all assays were 100%. GenomEra™ C. difficile had the highest retesting rate (12%). Simplexa™ C. difficile Direct benefits from both short hands-on time and total-turnaround time and 0% retesting. The differences in performance and accuracy between these three molecular assays are insignificant and all may be used as part of the routine algorithm for detection of C. difficile. |
| Databáze: | OpenAIRE |
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