An improved, highly sensitive method for the determination of serum iron using chromazurol B
| Autor: | Paolo Tarli, Paolo Neri, Alessandro Tabacco, Edoardo Moda |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
chemistry.chemical_classification
Ethanol Ligand Iron Biochemistry (medical) Clinical Biochemistry food and beverages Salt (chemistry) General Medicine Molar absorptivity Benzoates Biochemistry chemistry.chemical_compound chemistry Spectrophotometry Bromide Reagent Methods Humans Ammonium Ternary complex Chelating Agents Nuclear chemistry |
| Zdroj: | Clinica Chimica Acta. 114:287-290 |
| ISSN: | 0009-8981 |
| DOI: | 10.1016/0009-8981(81)90404-6 |
| Popis: | In a recent paper, Garlic [l] described the application of a new iron-chelating ligand, chromazurol B (CAB), in a manual method for the direct determination of serum iron and total iron-binding capacity. In this method, Fe(III)/Fe(II) reacts with CAB and cetyl trimethylammonium bromide (CTMA) to give a highly coloured ternary complex with a molar extinction coefficient of 1.68 X 10 5 1. mol ’ . cm’ at 630 nm. As this method, in our experience, gave a low percentage of recovery, we partially modified the original reagent composition and performed the reaction at 37°C instead of at room temperature. We also eliminated the preparation of CAB ammonium salt, by solubilizing the acid CAB directly in the reagent in the presence of ethanol, thereby obtaining a single, stable reagent. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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