Localization and nucleotide sequence of the gene for the membrane polypeptide D2 from pea chloroplast DNA

Autor: Knud W. Henningsen, Bjarne M. Stummann, O. F. Rasmussen, Gerhard Bookjans
Rok vydání: 2013
Předmět:
Zdroj: Plant molecular biology. 3(4)
ISSN: 0167-4412
Popis: The gene for the membrane polypeptide D2 has been mapped on the pea (Pisum sativum) chloroplast genome. The nucleotide sequence of the gene and its flanking regions is presented. The only large open reading frame in the sequence codes for a protein of MW 39.5 kD. A potential ribosome binding site is located 6 nucleotides upstream from the initiation codon and there are two sets of putative promotor sequences in the 5′ flanking region. The polypeptide has a high content of hydrophobic amino acids, predominatly grouped in clusters of 20 or more residues. The 3′ end of the D2 gene is overlapped by 50 nucleotides of a second open reading frame (UORF I) which is at least 369 nucleotides long. Based on current data we suggest the D2 polypeptide to be a constituent of photosystem II (PSII).
Databáze: OpenAIRE