The increasing hematopoietic effect of the combined treatment of Korean Red Ginseng and Colla corii asini on cyclophosphamide-induced immunosuppression in mice
| Autor: | Myung-Gyun Jeong, Evelyn Saba, Seung-Chun Park, Man Hee Rhee, Muhammad Irfan, Sung Dae Kim, Yi-Seong Kwak, Yixian Quah, Yuan Yee Lee |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Population Spleen Biology Biochemistry Genetics and Molecular Biology (miscellaneous) Andrology Blood cell 03 medical and health sciences 0302 clinical medicine Immune system medicine Colla corii asini education education.field_of_study immunosuppression Panax ginseng Botany hematopoiesis Haematopoiesis Pharmacology and Physiology 030104 developmental biology medicine.anatomical_structure Complementary and alternative medicine 030220 oncology & carcinogenesis QK1-989 cyclophosphamide Bone marrow Stem cell CD8 Biotechnology |
| Zdroj: | Journal of Ginseng Research, Vol 45, Iss 5, Pp 591-598 (2021) Journal of Ginseng Research |
| ISSN: | 1226-8453 |
| Popis: | Background Hematopoiesis is the production of blood cells from hematopoietic stem cells (HSCs) that reside in the bone marrow. Cyclophosphamide (CTX) is a chemotherapy drug that suppresses the immune system. Korean Red Ginseng (KRG) and Colla corii asini (CCA) have been traditionally used for boosting the immune system. Methods HSCs in the bone marrow, and immune cell subtype in splenocytes, PBMCs, and thymocytes were investigated. Serum levels of hematopoietic-related markers were analyzed using ELISA. Protein expression in spleen tissue was analyzed using western blot analysis. Hematoxylin & eosin staining in the femurs of mice were also conducted. Results The combination of KRG and CCA with a ratio of 3:2 increased HSCs, CD3 and CD8+ T cells in the circulation, and CD3 T cells in the spleen. A ratio of 2:3 (KRG:CCA) increased the thymic regulatory T cells and recovered the CD3 T cells in the spleen and circulation while recovering proteins in the JAK-STAT pathway in the spleen. Overall, blood cell population and differentiating factors vital for cell differentiation were also significantly recovered by all combinations especially in ratios of 3:2 and 2:3. Conclusion A ratio of 3:2 (KRG:CCA) is the most ideal combination as it recovered the HSC population in the bone marrow of mice. Graphical abstract Image 1 |
| Databáze: | OpenAIRE |
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