Subgingival areas as potential reservoirs of different Candida spp in type 2 diabetes patients and healthy subjects

Autor: Milena Radunovic, Ana Pucar, Valentina Arsić Arsenijević, Jovana Kuzmanovic Pficer, Milena Barac, Dušan Pavlica, Sanja Matić Petrović
Rok vydání: 2019
Předmět:
Male
0301 basic medicine
Gingiva
Yeast and Fungal Models
Candida glabrata
Pathology and Laboratory Medicine
Biochemistry
Gastroenterology
Candida tropicalis
Endocrinology
0302 clinical medicine
Oral Diseases
Candidiasis
Oral
Candida albicans
Medicine and Health Sciences
Diabetes diagnosis and management
Oral mucosa
Candida
Fungal Pathogens
Multidisciplinary
biology
Fungal Diseases
Eukaryota
Middle Aged
3. Good health
Infectious Diseases
medicine.anatomical_structure
Experimental Organism Systems
Medical Microbiology
Medicine
Female
Pathogens
Anatomy
Research Article
Adult
medicine.medical_specialty
HbA1c
Endocrine Disorders
Science
Oral Medicine
030106 microbiology
Mycology
Research and Analysis Methods
Microbiology
Periodontal curette
03 medical and health sciences
Tongue
Internal medicine
Diabetes Mellitus
medicine
Humans
Hemoglobin
Periodontitis
Microbial Pathogens
Periodontal Diseases
Mouth
business.industry
Organisms
Fungi
Biology and Life Sciences
Proteins
030206 dentistry
medicine.disease
biology.organism_classification
Chronic periodontitis
Yeast
Diagnostic medicine
Yeast Infections
Cross-Sectional Studies
Diabetes Mellitus
Type 2
Socioeconomic Factors
Metabolic Disorders
Chronic Periodontitis
Animal Studies
business
Digestive System
Zdroj: PLoS One
PLoS ONE, Vol 14, Iss 1, p e0210527 (2019)
PLoS ONE
Popis: ObjectivesThe aim of this cross-sectional observational study was to compare the prevalence of different oral Candida spp. in patients with Type 2 Diabetes and chronic periodontitis in two oral sites: dorsal surface of the tongue and subgingival area. In order to determine subgingival areas as potential reservoirs of yeasts, this study aimed to find differences in the yeasts' detection between the dorsum of the tongue, as the oral site most commonly inhabited with microorganisms, and subgingival samples. Additionally, potential predictors for the yeasts prevalence were determined.Material and methodsSubjects (N = 146) were divided into four groups: group A- healthy individuals without periodontitis, group B- healthy individuals with chronic periodontitis, group C- Type 2 Diabetes patients with good glycoregulation and Chronic periodontitis and group D- Type 2 Diabetes patients with poor glycoregulation and Chronic periodontitis. Samples were obtained from the tongue by swabbing. Subgingival plaque samples were taken by paper points and periodontal curette. Isolation and identification of different Candida spp. was done using ChromAgar medium. In addition, germ-tube production and carbohydrate assimilation tests were performed.ResultsThe prevalence of Candida spp. was higher in diabetics with poor glycoregulation. The most frequently isolated species was Candida albicans followed by Candida glabrata and Candida tropicalis. In 15.6% of cases, Candida spp. was present in the subgingival area while absent on the tongue. Multivariate regression model showed that HbA1c was Candida spp. predictor for both locations.ConclusionsOur results confirmed that there are Candida spp. carriers among subjects with clinically healthy oral mucosa. Also, this study identified subgingival areas as potential reservoirs of these pathogenic species. Glycoregulation has been recognized as a positive predictor factor of Candida spp.
Databáze: OpenAIRE
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