Synovial fluid T cells from patients with rheumatoid arthritis are refractory to the T helper type 2 differentiation‐inducing effects of interleukin‐4
| Autor: | O. Lassila, P. Isomäki, J. Punnonen, R. Luukkainen, P. Toivanen |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Adult
Male medicine.medical_treatment Immunology Enzyme-Linked Immunosorbent Assay Autoimmune Diseases Arthritis Rheumatoid Interferon-gamma Interleukin 21 Th2 Cells T-Lymphocyte Subsets Synovial Fluid Humans Immunology and Allergy Medicine Cytotoxic T cell Interferon gamma Interleukin 4 Aged business.industry Cell Differentiation T-Lymphocytes Helper-Inducer Original Articles Middle Aged Flow Cytometry Interleukin-12 Interleukin-10 Interleukin 10 Cytokine Interleukin 12 Cytokines Female Interleukin-4 business CD8 T-Lymphocytes Cytotoxic medicine.drug |
| Zdroj: | Immunology. 96:358-364 |
| ISSN: | 1365-2567 0019-2805 |
| Popis: | The balance between T helper type 1 (Th1) and Th2 cytokines is thought to be important in the initiation and outcome of autoimmune diseases. The goal of the present study was to compare the production of interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) by synovial fluid (SF) and peripheral blood (PB) CD4+ and CD8+ cells from patients with rheumatoid arthritis (RA) using three-colour immunofluorescence staining and flow cytometry, and to investigate the capacity of IL-4, IL-10 and IL-12 to modify the cytokine production profile of SF T cells. The frequency of IFN-gamma-producing CD4+ and CD8+ cells was significantly increased in SF when compared with PB. In contrast to IFN-gamma, the expression of IL-4 in SF and PB T cells was comparable. The majority of IL-4-producing cells in SF belonged to Th0/T cytotoxic (Tc) type 0 phenotype, whereas there were significantly more Th2/Tc2 cells in PB than in SF. Interestingly, IL-4 was unable to induce differentiation of non-adherent SF mononuclear cells (SFMC) into Th2 cells, whereas PB mononuclear cells (PBMC) under similar culture conditions differentiated into cells producing high levels of IL-4, IL-10 and IL-13. In contrast, there were no major differences in the effects of IL-10 and IL-12 on the cytokine production profile of SFMC when compared with PBMC. Taken together, the present results suggest that SF T cells from patients with RA are terminally differentiated into Th1/Tc1-like phenotype, and Th2/Tc2 differentiation-inducing agents, such as IL-4, may not be able to reverse the inflammatory process occurring in the joints. |
| Databáze: | OpenAIRE |
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