The Ability of Recombinant Murine Granulocyte-Macrophage Colony-Stimulating Factor to Protect Neonatal Rats from Septic Death Due to Staphylococcus aureus
| Autor: | E. S. Buescher, G. Sarman, R. w. Frenck, T. E. Harper |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Micrococcaceae
Neutrophils Granulocyte medicine.disease_cause Microbiology law.invention Leukocyte Count Colony-Stimulating Factors In vivo law Sepsis medicine Animals Immunology and Allergy Growth Substances biology Lethal dose Granulocyte-Macrophage Colony-Stimulating Factor Rats Inbred Strains Staphylococcal Infections biology.organism_classification Recombinant Proteins In vitro Rats Disease Models Animal Infectious Diseases medicine.anatomical_structure Granulocyte macrophage colony-stimulating factor Animals Newborn Staphylococcus aureus Recombinant DNA Granulocytes medicine.drug |
| Zdroj: | Journal of Infectious Diseases. 162:109-114 |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1093/infdis/162.1.109 |
| Popis: | Granulocyte-macrophage colony-stimulating factor (GM-CSF) potentiates in vitro and in vivo production of granulocytes. Also, recombinant human GM-CSF in vitro enhances functional capabilities of human granulocytes. Recombinant murine (rm) GM-CSF was administered to neonatal rats in vivo to test its ability to protect from septic death due to Staphylococcus aureus. When rmGM-CSF was given intraperitoneally 6 h before a 90% lethal dose challenge of S. aureus, peak survival was observed at a dose of 30 pg/g (54% vs. 10% in animals administered saline; P less than .001). Blood cultures were positive for S. aureus in 26 of 32 saline-treated and in 5 of 31 rmGM-CSF-treated animals (P less than .001). Numbers of blood granulocytes were significantly increased 9 h after administration of rmGM-CSF (30 pg/g) but returned to control levels by 12 h. Neither neutrophil storage nor proliferative pools were affected. Thus, rmGM-CSF significantly improved survival when given prophylactically in a neonatal rat model of infection. |
| Databáze: | OpenAIRE |
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