Stellate cells and mesenchymal stem cells in benign mammary stroma are associated with risk factors for breast cancer – an observational study

Autor: Johan Forsell, Håkan Olsson, Björn L Isfoss, Helena Jernström, Elin Sand, Bo Holmqvist
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Cancer Research
Pathology
Stem cells
Stem cell marker
Cancer risk
0302 clinical medicine
Breast cancer
Risk Factors
Breast
Aged
80 and over
BRCA1 Protein
Middle Aged
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Immunohistochemistry
3. Good health
Isoenzymes
Oncology
Risk factors for breast cancer
030220 oncology & carcinogenesis
Female
Stem cell
Research Article
Adult
medicine.medical_specialty
Stromal cell
Histology
Breast Neoplasms
lcsh:RC254-282
Aldehyde Dehydrogenase 1 Family
Familial cancer
03 medical and health sciences
Young Adult
Stroma
Genetics
medicine
Biomarkers
Tumor
Humans
Aged
BRCA2 Protein
business.industry
Mesenchymal stem cell
Retinal Dehydrogenase
Mesenchymal Stem Cells
Aldehyde dehydrogenase
medicine.disease
BRCA1
BRCA2
030104 developmental biology
Mutation
Hepatic stellate cell
business
Zdroj: BMC Cancer
BMC Cancer, Vol 18, Iss 1, Pp 1-13 (2018)
ISSN: 1471-2407
Popis: Background It is not known whether stromal cells in benign breast tissue can mediate risk of breast cancer. We recently described aldehyde dehydrogenase 1 A1 (ALDH1) positive (+) cells in morphologically normal breast stroma of premenopausal women, and the data indicated that their distribution is associated with clinical risk factors for breast cancer. The aim of the present study was to define the identities of these cells using histologic and immunohistologic methods, and to investigate associations between those cells and hormonal and genetic risk factors in pre- and postmenopausal women. Methods Stroma of morphologically normal tissue was analyzed in samples from 101 well-characterized women whose breasts had been operated. Morphology and immunolabeling were applied to determine cell identities based on the putative stem cell markers ALDH1 and stage-specific embryonic antigen-3 (SSEA3), and immunophenotypes indicating mast cells or stellate cells. The results were compared with the patients’ risk factors using regression analysis (two-tailed). Results ALDH1+ round/oval cells were associated with low parity in BRCA1/2 carriers (p = 0.022), while in non-BRCA1/2-carriers they were negatively associated with nulliparity (p = 0.057). In premenopausal women ALDH1+ round/oval cells were associated with family history (p = 0.058). SSEA3+ round/oval cells were morphologically and immunohistologically consistent with multilineage stress-enduring (Muse) cells, and these cells were independently associated with the breast cancer risk factors low parity (p = 0.015), family history (p = 0.021), and hormone use after menopause (p = 0.032). ALDH1+ spindle-shaped/polygonal cells were immunohistologically consistent with stellate cells, and were negatively associated with family history of breast cancer (p = 0.001). Conclusion This study identified novel stromal cell types in benign breast tissue that have a potential for stratifying women for breast cancer risk. Electronic supplementary material The online version of this article (10.1186/s12885-018-4151-x) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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