Fluorescence lifetime imaging of membrane lipid order with a ratiometric fluorescent probe
| Autor: | Vasyl Kilin, Oleksandr Glushonkov, Andrey S. Klymchenko, Yves Mély, Ludovic Richert, Lucas Herdly |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Membranes Chemistry Vesicle Membrane lipids Cell Membrane Analytical chemistry Biophysics Tautomer Fluorescence Cell membrane Membrane Lipids Membrane medicine.anatomical_structure Microscopy Fluorescence Intramolecular force medicine Humans Unilamellar Liposomes HeLa Cells |
| Zdroj: | Biophysical journal. 108(10) |
| ISSN: | 1542-0086 |
| Popis: | To monitor the lateral segregation of lipids into liquid-ordered (Lo) and -disordered (Ld) phases in lipid membranes, environment-sensitive dyes that partition in both phases but stain them differently have been developed. Of particular interest is the dual-color F2N12S probe, which can discriminate the two phases through the ratio of its two emission bands. These bands are associated with the normal (N∗) and tautomer (T∗) excited-state species that result from an excited-state intramolecular proton transfer. In this work, we investigated the potency of the time-resolved fluorescence parameters of F2N12S to discriminate lipid phases in model and cell membranes. Both the long and mean lifetime values of the T∗ form of F2N12S were found to differ by twofold between Ld and Lo phases as a result of the restriction in the relative motions of the two aromatic moieties of F2N12S imposed by the highly packed Lo phase. This differed from the changes in the ratio of the two emission bands between the two phases, which mainly resulted from the decreased hydration of the N∗ form in the Lo phase. Importantly, the strong difference in lifetimes between the two phases was preserved when cholesterol was added to the Ld phase. The two phases could be imaged with high contrast by fluorescence lifetime imaging microscopy (FLIM) on giant unilamellar vesicles. FLIM images of F2N12S-labeled live HeLa cells confirmed that the plasma membrane was mainly in the Lo-like phase. Furthermore, the two phases were found to be homogeneously distributed all over the plasma membrane, indicating that they are highly mixed at the spatiotemporal resolution of the FLIM setup. Finally, FLIM could also be used to sensitively monitor the change in lipid phase upon cholesterol depletion and apoptosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|