Selective depletion of chromosomes in a stable mouse-Chinese hamster hybrid cell line using antisera directed against species-specific cell surface antigens
| Autor: | Jeffrey J. Collins, Christopher J. Marshall, Ian Macpherson, Antonia T. Destree |
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| Rok vydání: | 1975 |
| Předmět: |
Antiserum
Immunoassay biology Physiology Immune Sera Clinical Biochemistry Cell Structural gene Cell Membrane Hamster Cell Biology Hybrid Cells biology.organism_classification Cytotoxicity Tests Immunologic Molecular biology Chinese hamster Chromosomes medicine.anatomical_structure Antigen Karyotyping medicine Cytotoxic T cell Antigens Gene |
| Zdroj: | Journal of cellular physiology. 86(3 Pt 2) |
| ISSN: | 0021-9541 |
| Popis: | A chromosomally-stable mouse-Chinese hamster hybrid cell line was subjected to five rounds of selection with cytotoxic antisera raised in rabbits against either the parental mouse 3T3 cells or the parental Chinese hamster Wg-1 cells. Routine karyological analysis of clones isolated at each stage of serum selection revealed that treatment with either serum resulted in a limited loss of chromosomes (compared to the untreated hybrid cell cultured in parallel) and that the pattern of chromosome loss could not be correlated with the particular antiserum used for selection. However, more detailed analysis with the SSC-formamide C-banding technique, which identifies chromosomes containing a mouse centromere region, demonstrated that while large-scale chromosome loss was not achieved as a result of antiserum selection, the limited loss of chromosomes did, in fact, reflect a specific depletion of chromosomes in response to treatment with cytotoxic antiserum. Specific chromosomal elimination was shown to occur as early as the first round of antiserum treatment. Antigenic analysis of the serum-selected clones revealed a quantitative decrease in the expression of the species-specific surface antigens selected against, but no qualitative loss of antigens was detected. The results suggest that treatment with cytotoxic antiserum may select for clones that have lost specific chromosomes bearing genes regulating the expression of species-specific surface antigens, rather than for those demonstrating large-scale depletion of chromosomes bearing the corresponding structural genes. |
| Databáze: | OpenAIRE |
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