Cell tropism of the simian foamy virus type 1 (SFV-1)
| Autor: | Ayalew Mergia, Nancy J. Leung, Jeanne Blackwell |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Primates
Cell type T-Lymphocytes viruses Molecular Sequence Data Biology Virus Replication Polymerase Chain Reaction Quail Virus Cell Line Species Specificity medicine Animals Humans Macrophage Fluorescent Antibody Technique Indirect Fibroblast Tropism DNA Primers B-Lymphocytes Base Sequence General Veterinary Macrophages RNA-Directed DNA Polymerase biochemical phenomena metabolism and nutrition Virology Molecular biology Reverse transcriptase medicine.anatomical_structure Cell culture Cytopathology DNA Viral Vertebrates Spumavirus Animal Science and Zoology |
| Zdroj: | Journal of Medical Primatology. 25:2-7 |
| ISSN: | 0047-2565 |
| DOI: | 10.1111/j.1600-0684.1996.tb00185.x |
| Popis: | Several cell lines representing different species and cell types were tested for simian foamy virus type 1 (SFV-1) infection. SFV-1 infections were monitored by polymerase chain reaction, reverse transcriptase, cytopathology, and immunofluorescent assays. All cells tested were permissive for SFV-1, demonstrating that SFV-1 has a broad host range with respect to species and cell types. Infected fibroblasts, epithelial cells, and neural cells all showed extensive cytopathology that is characteristic of foamy virus infection. No cytopathology was induced in T cell-derived Jurkats and Hut-78 cell lines. The cytopathic effects in B and macrophage originated cells were delayed by several days. Cytopathology in these cell lines was not as dramatic as seen in the infected fibroblast and epithelial cells. The reverse transcriptase values in the SFV-1 infected lymphoid and macrophage cell lines were severalfold lower than that of the fibroblasts and epithelial cells. Therefore, SFV-1 appears to establish a low level persistent infection in lymphoid and macrophage cell lines. |
| Databáze: | OpenAIRE |
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