Glypican-1 in human glioblastoma: implications in tumorigenesis and chemotherapy
| Autor: | Eduardo Listik, Leny Toma |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
chemotherapy resistance temozolomide medicine.disease_cause 03 medical and health sciences 0302 clinical medicine medicine Lipid raft Temozolomide biology Cell growth glioblastoma Cell migration glypican-1 In vitro tumorigenesis 030104 developmental biology Oncology Proteoglycan Cell culture 030220 oncology & carcinogenesis biology.protein Cancer research Carcinogenesis Research Paper medicine.drug |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| Popis: | Glioblastoma is one of the most common malignant brain tumors, with which patients have a mean survival of 24 months. Glypican-1 has been previously shown to be overexpressed in human glioblastoma and to be negatively correlated with patient’s survival. This study aimed to investigate how glypican-1 influences the tumoral profile of human glioblastoma using in vitro cell line models. By downregulating the expression of glypican-1 in U-251 MG cells, we observed that the cellular growth and proliferation were highly reduced, in which cells were significantly shifted towards G0 as opposed to G1 phases. Cellular migration was severely affected, and glypican-1 majorly impacted the affinity towards laminin-binding of glioblastoma U-251 MG cells. This proteoglycan was highly prevalent in glioblastoma cells, being primarily localized in the cellular membrane and extracellular vesicles, occasionally with glypican-3. Glypican-1 could also be found in cell-cell junctions with syndecan-4 but was not identified in lipid rafts in this study. Glypican-1-silenced cells were much more susceptible to temozolomide than in U-251 MG itself. Therefore, we present evidence not only to support facts that glypican-1 is an elementary macromolecule in glioblastoma tumoral microenvironment but also to introduce this proteoglycan as a promising therapeutic target for this lethal tumor. |
| Databáze: | OpenAIRE |
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