Expression of TOB/BTG family members in patients with inflammatory bowel disease
| Autor: | Janette Furuzawa-Carballeda, Braulio Martínez-Benítez, Angel Priego-Ranero, Gabriela Fonseca-Camarillo, Jesús K. Yamamoto-Furusho, Rafael Barreto-Zuñiga |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
Male 0301 basic medicine Colon T cell Immunology Gene Expression Connective tissue Inflammation Inflammatory bowel disease Immediate-Early Proteins 03 medical and health sciences 0302 clinical medicine Gene expression Humans Medicine RNA Messenger Intestinal Mucosa Cell Proliferation BTG2 business.industry Macrophages Tumor Suppressor Proteins Receptors IgG Epithelial Cells General Medicine Middle Aged Colitis Inflammatory Bowel Diseases medicine.disease Cross-Sectional Studies Ki-67 Antigen 030104 developmental biology medicine.anatomical_structure Cancer research Immunohistochemistry Female medicine.symptom business BTG1 030215 immunology |
| Zdroj: | Scandinavian Journal of Immunology. 93 |
| ISSN: | 1365-3083 0300-9475 |
| Popis: | In recent years, the role of anti-proliferative TOB proteins in the regulation of immune response by inhibiting T cell activation has been demonstrated. Nevertheless, no previous studies have explored their expression in patients with IBD. The aim of the study was to characterize the gene and protein expression of the TOB/BTG family in intestinal tissue of patients with IBD. This is an observational and cross-sectional study that included 63 IBD patients. Gene expression of TOB/BTG family was measured by RT-PCR. Protein expression of TOB/CD16 and BTG/Ki-67 was evaluated by immunohistochemistry. TOB/BTG family mRNAs were detected and quantitated by RT-qPCR in rectal and ileum biopsies from UC patients and CD patients, respectively, and non-inflammatory control tissues. Results showed that TOB1 and BTG1 gene expression was decreased in the colonic mucosa from patients with UC compared with the control group. The TOB2 and BTG2 genes were over-expressed in the colonic mucosa of patients with UC in remission compared with the active UC and control group. The high TOB2 gene expression was associated with histological remission (P = .01). TOB1/CD16, TOB2/CD16, BTG1/Ki-67, BTG2/Ki-67 and BTG4/Ki-67 single and double positive cells were mostly NK, macrophages, epithelial cells, connective tissue cells and perivascular inflammatory infiltrates in tissues from patients with UC and CD. This is the first depiction of the TOB/BTG family gene and protein expression in rectal and ileum tissues by a CD16+ subpopulation in IBD. |
| Databáze: | OpenAIRE |
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