Pharmacological characterization of α1-adrenoceptors in mouse isolated femoral small arteries
| Autor: | Chris Hillier, Joseph Zacharia, Allan MacDonald |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Male
medicine.medical_specialty Stimulation In Vitro Techniques Clonidine Piperazines Norepinephrine (medication) Mice Norepinephrine chemistry.chemical_compound Chloroethylclonidine Receptors Adrenergic alpha-1 Internal medicine medicine Prazosin Animals Vasoconstrictor Agents Adrenergic alpha-Antagonists Pharmacology Dose-Response Relationship Drug Chemistry Electric Stimulation Femoral Artery Endocrinology medicine.anatomical_structure Mice Inbred DBA Vasoconstriction Circulatory system Adrenergic alpha-1 Receptor Antagonists Catecholamine Vascular Resistance Adrenergic alpha-Agonists Algorithms medicine.drug Myograph Blood vessel |
| Zdroj: | European Journal of Pharmacology. 503:155-163 |
| ISSN: | 0014-2999 |
| DOI: | 10.1016/j.ejphar.2004.09.046 |
| Popis: | Arteries were isolated from male DBA/2 mice and mounted on a small vessel wire myograph for isometric recording. Responses to exogenous noradrenaline were inhibited with high affinity by prazosin (pKB, 9.3) and 5-methyl-urapidil (pKB, 9.2) and with low affinity by 8-[2-[4-(2-methoxyphenyl)-1 piperazinyl]ethyl]-8-azaspiro[4.5]decane-7,9-dione (BMY 7378) (pA(2), 6.7). Chloroethylclonidine (10 microM) produced only a small reduction in the maximum response to noradrenaline. Responses to electrical field stimulation were also inhibited with high affinity by prazosin (pIC50, 9.3-9.5) and 5-methyl-urapidil (pIC50, 8.0-8.3). Responses were sensitive to BMY 7378 at low frequencies of stimulation (pIC50 at 2 Hz, 8.2) but not at high frequencies (pIC50 at 20 Hz, 6.5). In conclusion, contractions to exogenous and endogenous noradrenaline in mouse femoral small arteries are mediated mainly by alpha1A-adrenoceptors. alpha1D-adrenoceptors are not involved in responses to exogenous noradrenaline but appear to be activated by neurally released noradrenaline at a low frequency of stimulation. |
| Databáze: | OpenAIRE |
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