Modifying Effect of Autotransfusion of Mesenchymal Stromal Cells on the Production of Reactive Oxygen Species and Cytokines by Mononuclear Cells in Patients with Chronic Heart Failure

Autor: A. G. Konoplyannikov, V. N. Petrov, I. V. Semenkova, E. V. Agaeva, E. V. Sayapina, O. E. Popovkina, M. A. Kaplan, L. A. Lepekhina
Rok vydání: 2017
Předmět:
0301 basic medicine
Lipopolysaccharides
Stromal cell
Primary Cell Culture
Mesenchymal Stem Cell Transplantation
Peripheral blood mononuclear cell
Transplantation
Autologous
General Biochemistry
Genetics and Molecular Biology
Dinoprostone
03 medical and health sciences
Interferon-gamma
0302 clinical medicine
In vivo
Interleukin-1alpha
Medicine
Humans
Lymphotoxin-alpha
chemistry.chemical_classification
Heart Failure
Reactive oxygen species
business.industry
Tumor Necrosis Factor-alpha
Mesenchymal stem cell
Granulocyte-Macrophage Colony-Stimulating Factor
Mesenchymal Stem Cells
General Medicine
In vitro
Interleukin-10
030104 developmental biology
chemistry
Gene Expression Regulation
Immunology
Leukocytes
Mononuclear
Tumor necrosis factor alpha
business
Reactive Oxygen Species
030217 neurology & neurosurgery
Autotransfusion
Signal Transduction
Zdroj: Bulletin of experimental biology and medicine. 164(2)
ISSN: 1573-8221
Popis: We studied in vivo modifying effect of autotransfusion of human bone marrow mesenchymal stromal cells on ROS generation and production of cytokines (TNFα,TNFβ, IL-1α, IL-10, IFNγ, and GM-CSF) and PGE2 by mononuclear cells of patients (N=21) with chronic heart failure. These parameters were evaluated prior to (control) and after (immediately and on day 14) intravenous administration of stromal cells in doses of 100-200×106. Immediately after autotransfusion, significant increase of in vitro zymosan-induced chemiluminescence of blood mononuclear cells from 10 patients was observed. At later terms after autotransfusion (day 14), inhibition of chemiluminescent activity of blood mononuclear cells was revealed in 50% patients. We discuss possible mechanisms of involvement of transplanted autologous bone marrow mesenchymal stromal cells in reprogramming of blood mononuclear phagocytes from the pro- to anti-inflammatory phenotype under conditions of their in vivo interaction manifesting in transition from activation to inhibition of ROS-producing activity of macrophages and significant suppression of in vitro LPS-induced production of TNFα and GM-CSF by blood mononuclears against the background of significantly elevated TNFβ, IL-10, and IL-1α concentrations.
Databáze: OpenAIRE
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