Comparative developmental expression profiling of two C. elegans isolates

Autor: Sonja M. Skrovanek, Emily J. Capra, Leonid Kruglyak
Přispěvatelé: Wu, Xiaolin
Rok vydání: 2008
Předmět:
General Science & Technology
1.1 Normal biological development and functioning
ved/biology.organism_classification_rank.species
lcsh:Medicine
Biology
03 medical and health sciences
0302 clinical medicine
Genetic
Underpinning research
Gene expression
Genetic variation
Genetics
Animals
Innate
Developmental
Polymorphism
lcsh:Science
Model organism
Caenorhabditis elegans
Gene
030304 developmental biology
Oligonucleotide Array Sequence Analysis
Regulation of gene expression
0303 health sciences
Multidisciplinary
Polymorphism
Genetic
ved/biology
Gene Expression Profiling
lcsh:R
Immunity
Gene Expression Regulation
Developmental
Genetic Variation
Genetics and Genomics/Gene Expression
biology.organism_classification
Immunity
Innate
Gene expression profiling
Gene Expression Regulation
lcsh:Q
Generic health relevance
DNA microarray
030217 neurology & neurosurgery
Research Article
Developmental Biology
Computational Biology/Genomics
Biotechnology
Zdroj: PloS one, vol 3, iss 12
PLoS ONE
PLoS ONE, Vol 3, Iss 12, p e4055 (2008)
Popis: Gene expression is known to change during development and to vary among genetically diverse strains. Previous studies of temporal patterns of gene expression during C. elegans development were incomplete, and little is known about how these patterns change as a function of genetic background. We used microarrays that comprehensively cover known and predicted worm genes to compare the landscape of genetic variation over developmental time between two isolates of C. elegans. We show that most genes vary in expression during development from egg to young adult, many genes vary in expression between the two isolates, and a subset of these genes exhibit isolate-specific changes during some developmental stages. This subset is strongly enriched for genes with roles in innate immunity. We identify several novel motifs that appear to play a role in regulating gene expression during development, and we propose functional annotations for many previously unannotated genes. These results improve our understanding of gene expression and function during worm development and lay the foundation for linkage studies of the genetic basis of developmental variation in gene expression in this important model organism.
Databáze: OpenAIRE
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