Prevalence and molecular epidemiology of ESBLs, plasmid-determined AmpC-type β-lactamases and carbapenemases among diarrhoeagenic Escherichia coli isolates from children in Gwangju, Korea: 2007-16
| Autor: | Kwang gon Kim, Ji Hyun Shin, Hye Jung Park, Jae Keun Chung, Hye young Kee, Min-Ji Kim, Duck Woong Park, Jin Jeong |
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| Rok vydání: | 2019 |
| Předmět: |
Microbiology (medical)
DNA Bacterial Diarrhea Male Cefotaxime Gene Transfer Horizontal Microbial Sensitivity Tests Biology medicine.disease_cause beta-Lactamases Microbiology Bacterial Proteins Drug Resistance Multiple Bacterial Genotype Republic of Korea Pulsed-field gel electrophoresis medicine Escherichia coli Prevalence Humans Pharmacology (medical) Child Escherichia coli Infections Pharmacology Molecular epidemiology Outbreak biology.organism_classification Enterobacteriaceae Hospitals Anti-Bacterial Agents Electrophoresis Gel Pulsed-Field Infectious Diseases Carriage Child Preschool Female medicine.drug |
| Zdroj: | The Journal of antimicrobial chemotherapy. 74(8) |
| ISSN: | 1460-2091 |
| Popis: | Objectives Young children could act as important carriers of cefotaxime-resistant Enterobacteriaceae. However, most studies on these bacteria have focused on hospitalized adults. Therefore, we determined the prevalence and characteristics of ESBL-, plasmid-determined AmpC-type β-lactamase (PABL)- and carbapenemase-producing diarrhoeagenic Escherichia coli isolates mainly from infants and children in the south-west region of Korea over a 10 year period. Methods Non-duplicate E. coli clinical isolates were recovered from diarrhoeagenic patient specimens at 12 hospitals in Gwangju, Korea, between January 2007 and December 2016. Antimicrobial susceptibilities and molecular features of ESBL- and carbapenemase-producing isolates were determined. Results A total of 1047 pathogenic E. coli isolates were collected and 58 cefotaxime-resistant E. coli isolates (5.5%) were identified. The prevalence and types of β-lactamase genes increased steadily from 5.7% in 2007 to 11.6% in 2016 with some fluctuations. CTX-M-14 (53.4%) was the predominant CTX-M genotype. PFGE revealed high genetic heterogeneities among diarrhoeagenic E. coli isolates, suggesting horizontal transfer of antibiotic resistance genes, which was also proved by conjugation assay. Conclusions Progressive increases in carriage rates and the number of β-lactamase types, and the possibility of community outbreaks of these food-borne bacteria in young children, may pose tangible public health threats. |
| Databáze: | OpenAIRE |
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