Generation of Superparamagnetic Liposomes Revealed as Highly Efficient MRI Contrast Agents for in Vivo Imaging
| Autor: | Marie-Sophie Martina, Cécile Grabielle-Madelmont, Sylviane Lesieur, Gillian Barratt, Jean-Paul Fortin, Olivier Clément, Florence Gazeau, Christine Ménager, Valérie Cabuil |
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| Přispěvatelé: | Physico-chimie, pharmacotechnie, biopharmacie (PCPB), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Matière et Systèmes Complexes (MSC (UMR_7057)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Liquides Ioniques et Interfaces Chargées (LI2C), Centre National de la Recherche Scientifique (CNRS)-ESPCI ParisTech-Université Pierre et Marie Curie - Paris 6 (UPMC), Imagerie médicale et quantitative, Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7), Université Pierre et Marie Curie - Paris 6 (UPMC)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Matière et Systèmes Complexes (MSC), This work was supported by a grant of the Ministry of Research in France, by CNRS and ACI NR 145 (ACI Nanoscience et Nanotechnologie et Programme Interdisciplinaire Imagerie du Petit Animal). |
| Rok vydání: | 2005 |
| Předmět: |
Ferrofluid
[SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging Iron Iron oxide Contrast Media 02 engineering and technology [CHIM.INOR]Chemical Sciences/Inorganic chemistry 010402 general chemistry 01 natural sciences Biochemistry Catalysis Polyethylene Glycols Magnetics Mice chemistry.chemical_compound Colloid and Surface Chemistry Nuclear magnetic resonance Drug Stability Cell Line Tumor Phosphatidylcholine Animals [PHYS.MECA.BIOM]Physics [physics]/Mechanics [physics]/Biomechanics [physics.med-ph] ComputingMilieux_MISCELLANEOUS Liposome Chemistry Macrophages Vesicle Oxides General Chemistry 021001 nanoscience & nanotechnology Magnetic Resonance Imaging Ferrosoferric Oxide Nanostructures 0104 chemical sciences Ferumoxytol Liposomes Phosphatidylcholines 0210 nano-technology [PHYS.COND.CM-SCM]Physics [physics]/Condensed Matter [cond-mat]/Soft Condensed Matter [cond-mat.soft] Ethylene glycol Magnetic Resonance Angiography Superparamagnetism |
| Zdroj: | Journal of the American Chemical Society Journal of the American Chemical Society, American Chemical Society, 2005, 127 (30), pp.10676-10685 Journal of the American Chemical Society, 2005, 127 (30), pp.10676-10685. ⟨10.1021/ja0516460⟩ |
| ISSN: | 1520-5126 0002-7863 |
| Popis: | International audience; Maghemite (γ-Fe2O3) nanocrystals stable at neutral pH and in isotonic aqueous media were synthesized and encapsulated within large unilamellar vesicles of egg phosphatidylcholine (EPC) and distearoyl-SN-glycero-3-phosphoethanolamine-N-[methoxy(poly(ethylene glycol))-2000] (DSPE-PEG2000, 5 mol %), formed by film hydration coupled with sequential extrusion. The nonentrapped particles were removed by flash gel exclusion chromatography. The magnetic-fluid-loaded liposomes (MFLs) were homogeneous in size (195 ± 33 hydrodynamic diameters from quasi-elastic light scattering). Iron loading was varied from 35 up to 167 Fe(III)/lipid mol %. Physical and superparamagnetic characteristics of the iron oxide particles were preserved after liposome encapsulation as shown by cryogenic transmission electron microscopy and magnetization curve recording. In biological media, MFLs were highly stable and avoided ferrofluid flocculation while being nontoxic toward the J774 macrophage cell line. Moreover, steric stabilization ensured by PEG-surface-grafting significantly reduced liposome association with the macrophages. The ratios of the transversal (r2) and longitudinal (r1) magnetic resonance (MR) relaxivities of water protons in MFL dispersions (6 < r2/r1 < 18) ranked them among the best T2 contrast agents, the higher iron loading the better the T2 contrast enhancement. Magnetophoresis demonstrated the possible guidance of MFLs by applying a magnetic field gradient. Mouse MR imaging assessed MFLs efficiency as contrast agents in vivo: MR angiography performed 24 h after intravenous injection of the contrast agent provided the first direct evidence of the stealthiness of PEG-ylated magnetic-fluid-loaded liposomes. |
| Databáze: | OpenAIRE |
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