Oral cavity tumors in younger patients show a poor prognosis and do not contain viral RNA
Autor: | Kathryn T. Bieging, Arun Khattri, Christopher D. Brown, Thomas Stricker, Everett E. Vokes, Ezra E.W. Cohen, Johannes Brägelmann, Tanguy Y. Seiwert, Mark W. Lingen, Kenneth R. Alexander, Megan E. McNerney, Zhixiang Zuo, M. El Dinali, Ravi Salgia, Kevin P. White, Michaela K. Keck, Masha Kocherginsky, Richard Longnecker, Ibiayi Dagogo-Jack |
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Rok vydání: | 2013 |
Předmět: |
Adult
Male Oncology Herpesvirus 4 Human Cancer Research medicine.medical_specialty Pathology Tongue Internal medicine medicine Humans Oral Cavity Squamous Cell Carcinoma Papillomaviridae Survival analysis Cervical cancer business.industry Incidence (epidemiology) Head and neck cancer Prognosis medicine.disease Survival Analysis Lymphoma medicine.anatomical_structure RNA Viral Female Mouth Neoplasms Oral Surgery business Algorithms Oncovirus |
Zdroj: | Oral Oncology. 49:525-533 |
ISSN: | 1368-8375 |
Popis: | summary Background: Oral cavity and in particular oral tongue cancers occur with a rising incidence in younger patients often lacking the typical risk factors of tobacco use, alcohol use, and human papilloma virus (HPV) infection. Their prognosis when treated with chemoradiation has not been well studied and responsible risk factors remain elusive. A viral etiology (other than HPV) has been hypothesized. Methods: First we analyzed outcomes from 748 head and neck cancer patients with locoregionally advanced stage tumors treated with curative-intent chemoradiation by anatomic site. Second, we analyzed seven oral tongue (OT) tumors from young, non-smokers/non-drinkers for the presence of viral mRNA using short-read massively-parallel sequencing (RNA-Seq) in combination with a newly-developed digital subtraction method followed by viral screening and discovery algorithms. For positive controls we used an HPV16-positive HNC cell line, a cervical cancer, and an EBV-LMP2A transgene lymphoma. Results: Younger patients with oral cavity tumors had worse outcomes compared to non-oral cavity patients. Surprisingly none of the seven oral tongue cancers showed significant presence of viral transcripts. In positive controls the expected viral material was identified. Conclusion: Oral cavity tumors in younger patients have a poor prognosis and do not appear to be caused by a transcriptionally active oncovirus. |
Databáze: | OpenAIRE |
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