Effects of an EPSPS-transgenic soybean line ZUTS31 on root-associated bacterial communities during field growth
| Autor: | Jing Cheng, Li-Ya Zhang, Gu-Hao Wang, Huixia Shou, Gui-Hua Lu, Cheng-Yi Tang, Jin-Liang Qi, Yong-Hua Yang, Yin-Ling Zhu, Xiao-Mei Hua |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Beta diversity lcsh:Medicine Plant Science Genetically modified crops Genetically Modified Plants 01 natural sciences Genetically Modified Crops lcsh:Science Data Management Rhizosphere Multidisciplinary Ecology Genetically Modified Organisms Bacterial taxonomy Eukaryota food and beverages Agriculture Plants Genetic Engineering Research Article Biotechnology Microbial Taxonomy Computer and Information Sciences Crops Biology Rhizobacteria Microbiology Bradyrhizobium 03 medical and health sciences Symbiosis Plant-Environment Interactions Botany Plant Communities Taxonomy Bacteria Plant Ecology lcsh:R Ecology and Environmental Sciences Bacterial Taxonomy Organisms Biology and Life Sciences Bacteriology biology.organism_classification Species Interactions 030104 developmental biology lcsh:Q Plant Biotechnology Alpha diversity Soybean Crop Science 010606 plant biology & botany |
| Zdroj: | PLoS ONE PLoS ONE, Vol 13, Iss 2, p e0192008 (2018) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0192008 |
| Popis: | The increased worldwide commercial cultivation of transgenic crops during the past 20 years is accompanied with potential effects on the soil microbial communities, because many rhizosphere and endosphere bacteria play important roles in promoting plant health and growth. Previous studies reported that transgenic plants exert differential effects on soil microbial communities, especially rhizobacteria. Thus, this study compared the soybean root-associated bacterial communities between a 5-enolpyruvylshikimate-3-phosphate synthase -transgenic soybean line (ZUTS31 or simply Z31) and its recipient cultivar (Huachun3 or simply HC3) at the vegetative, flowering, and seed-filling stages. High-throughput sequencing of 16S rRNA gene (16S rDNA) V4 hypervariable region amplicons via Illumina MiSeq and real-time quantitative PCR (qPCR) were performed. Our results revealed no significant differences in the overall alpha diversity of root-associated bacterial communities at the three developmental stages and in the beta diversity of root-associated bacterial communities at the flowering stage between Z31 and HC3 under field growth. However, significant differences in the beta diversity of rhizosphere bacterial communities were found at the vegetative and seed-filling stages between the two groups. Furthermore, the results of next generation sequencing and qPCR showed that the relative abundances of root-associated main nitrogen-fixing bacterial genera, especially Bradyrhizobium in the roots, evidently changed from the flowering stage to the seed-filling stage. In conclusion, Z31 exerts transitory effects on the taxonomic diversity of rhizosphere bacterial communities at the vegetative and seed-filling stages compared to the control under field conditions. In addition, soybean developmental change evidently influences the main symbiotic nitrogen-fixing bacterial genera in the roots from the flowering stage to the seed-filling stage. |
| Databáze: | OpenAIRE |
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