Evaluation of Potential Environmental Contamination Sources for the Presence of Multidrug-Resistant Bacteria Linked to Wound Infections in Combat Casualties
| Autor: | David W Cole, Edward F. Keen, Bernadette L. Thompson, Timothy E. Wallum, Joel T. Rose, Heather C. Yun, Katrin Mende, Helen K. Crouch, Clinton K. Murray, Matthew E. Griffith, Charles H. Guymon, Wade K. Aldous |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Microbiology (medical)
Operating Rooms Warfare Epidemiology Air Microbiology Drug resistance Hospitals Military medicine.disease_cause Microbiology Drug Resistance Multiple Bacterial Gram-Negative Bacteria medicine Humans Infection control Soil Microbiology Aerosolization Aerosols Cross Infection Infection Control biology business.industry Afghanistan Contamination biology.organism_classification United States Anti-Bacterial Agents Infectious Diseases Surgical Procedures Operative Iraq Equipment Contamination Acinetobacter lwoffii business Staphylococcus Soil microbiology Mobile Health Units |
| Zdroj: | Infection Control & Hospital Epidemiology. 33:905-911 |
| ISSN: | 1559-6834 0899-823X 0195-9417 |
| DOI: | 10.1086/667382 |
| Popis: | Objective.To determine whether multidrug-resistant (MDR) gram-negative organisms are present in Afghanistan or Iraq soil samples, contaminate standard deployed hospital or modular operating rooms (ORs), or aerosolize during surgical procedures.Design.Active surveillance.Setting.US military hospitals in the United States, Afghanistan, and Iraq.Methods.Soil samples were collected from sites throughout Afghanistan and Iraq and analyzed for presence of MDR bacteria. Environmental sampling of selected newly established modular and deployed OR high-touch surfaces and equipment was performed to determine the presence of bacterial contamination. Gram-negative bacteria aerosolization during OR surgical procedures was determined by microbiological analysis of settle plate growth.Results.Subsurface soil sample isolates recovered in Afghanistan and Iraq included various pansusceptible members of Enterobacteriaceae,Vibriospecies,Pseudomonasspecies,Acinetobacter Iwojfii, and coagulase-negativeStaphylococcus(CNS). OR contamination studies in Afghanistan revealed 1 surface with aMicrococcus luteus. Newly established US-based modular ORs and the colocated fixed-facility ORs revealed no gram-negative bacterial contamination prior to the opening of the modular OR and 5 weeks later. Bacterial aerosolization during surgery in a deployed fixed hospital revealed a mean gram-negative bacteria colony count of 12.8 colony-forming units (CFU)/dm2/h (standard deviation [SD], 17.0) during surgeries and 6.5 CFU/dm2/h (SD, 7.5;P= .14) when the OR was not in use.Conclusion.This study demonstrates no significant gram-negative bacilli colonization of modular and fixed-facility ORs or dirt and no significant aerosolization of these bacilli during surgical procedures. These results lend additional support to the role of nosocomial transmission of MDR pathogens or the colonization of the patient themselves prior to injury. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|