Direct sequencing of the white spot syndrome virus from Brazil: Genome assembly and new insights on phylogeny
| Autor: | Paulo Eduardo T. Soares, João Paulo Matos Santos Lima, Daniel Carlos Ferreira Lanza, Márcia Danielle A. Dantas, Diego Gomes Teixeira, Rita C. B. Silva-Portela, Lucymara Fassarella Agnez-Lima |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research Genes Viral White spot syndrome Sequence assembly Genome Viral Genome Virus Shrimp farming 03 medical and health sciences White spot syndrome virus 1 Genome Size Penaeidae Phylogenetics Virology Whiteleg shrimp Animals Homologous Recombination Mexico Phylogeny Genetics biology Phylogenetic tree Chromosome Mapping Molecular Sequence Annotation Sequence Analysis DNA biology.organism_classification Thailand Biological Evolution 030104 developmental biology Infectious Diseases Gene Ontology DNA Viral Brazil |
| Zdroj: | Virus research. 245 |
| ISSN: | 1872-7492 |
| Popis: | White spot syndrome virus (WSSV) has been the cause of great economic losses in world shrimp farming. In this work the genome of a Brazilian WSSV isolate was determined from direct sequencing of total DNA extracted from an infected whiteleg shrimp, and assembled based on a chimera template approach. Comparisons between WSSV-BR and other isolates revealed that the Brazilian virus has a relatively small genome, and is very similar to isolates from Thailand and Mexico. A phylogenetic relationship using different approaches has demonstrated that these isolates share a common evolutionary history. An analysis of conflicting phylogenetic signals also considering genomes of other isolates revealed that the evolutionary history of WSSV may be related to recombination events. We observed that these events can also be traced at some level by analyzing the homologous regions in the WSSV genome. The existence of recombination events introduces a new point of view that must be considered in the evolutionary history of WSSV. |
| Databáze: | OpenAIRE |
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