Bacteriological and mechanical impact of the Sterrad sterilization method on personalized 3D printed guides for mandibular reconstruction
| Autor: | Paola Aguilar, Jean-Paul Meningaud, Ronan Leguen, Céline Leplay, Paul L. Woerther, Moussa Oudjhani, Lionel Tortolano, Romain Bosc, Barbara Hersant |
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| Rok vydání: | 2021 |
| Předmět: |
Models
Anatomic 0209 industrial biotechnology 3d printed Materials science Surface Properties Science Acrylic Resins 3d model Mandible 02 engineering and technology Article 3d printer 03 medical and health sciences 020901 industrial engineering & automation 0302 clinical medicine 3-D reconstruction Butadienes Humans Implants Mandibular reconstruction Multidisciplinary Bacteria Log reduction Atomic force microscopy Mechanical impact Sterilization Sterilization (microbiology) Surgical Instruments 030220 oncology & carcinogenesis Printing Three-Dimensional Equipment Contamination Polystyrenes Medicine Mandibular Reconstruction Reconstruction Hydrophobic and Hydrophilic Interactions Biomedical engineering |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-10 (2021) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-020-79752-7 |
| Popis: | Surgical cutting guides are increasingly used for maxillofacial reconstruction. They are usually provided by laboratories. In recent years, surgical teams have published studies on the possibility of manufacturing their own cutting guides thanks to 3D printers. The object of this study is to analyze the impact of the sterilization on the surface of those personalized models and to assess the effectiveness of sterilization. Using the data from high-resolution CT scan of patient, 3D models were generated through computerized assisted design and fabricated with a 3D printer using Acrylonitrile Butadiene Styrene (ABS). For the sterilization, a Sterrad method was used. In order to evaluate the effectiveness of sterilization, 3D models were artificially contaminated with several bacterial reference strains, sterilized and finally cultured. The surfaces and mechanical modifications were analyzed before and after sterilization with infrared spectrometry, surface contact angle, extensometer, scanning electron microscopy and atomic force microscopy. Ten models of different shapes and 24 samples were fabricated, sterilized and analyzed. The 3D models were designed in 48 h, printed in an average of 122 min and underwent a 47 min cycle of sterilization. All experimentally contaminated 3D models were negative in culture, with at least, a six log reduction of the initial inoculum. The hydrophobicity and roughness of the surface suffered few changes. The reproducibility of this procedure was proved by identical results in the three sterilization rounds. Using Sterrad process for the sterilization of ABS printed material doesn’t represent a bacterial risk for the patient. It is a feasible and safe innovative reconstructive method that can save time particularly for oncological cases. |
| Databáze: | OpenAIRE |
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