Penetrating Peptide-Bioconjugated Persistent Nanophosphors for Long-Term Tracking of Adipose-Derived Stem Cells with Superior Signal-to-Noise Ratio
| Autor: | Cheng-Xiong Yang, Shu-Qi Wu, Chong-Wei Chi, Xiu-Ping Yan |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Luminescence Infrared Rays Cellular differentiation Nanotechnology Mice Transgenic 02 engineering and technology Signal-To-Noise Ratio 010402 general chemistry 01 natural sciences Analytical Chemistry Mice Persistent luminescence In vivo Animals Cells Cultured Cell Proliferation Chemistry Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells 021001 nanoscience & nanotechnology 0104 chemical sciences Mice Inbred C57BL Autofluorescence Adipose Tissue Cell Tracking Nanoparticles Stem cell 0210 nano-technology Preclinical imaging Biomedical engineering |
| Zdroj: | Analytical chemistry. 88(7) |
| ISSN: | 1520-6882 |
| Popis: | Reliable long-term in vivo tracking of stem cells is of great importance in stem cell-based therapy and research. Fluorescence imaging with in situ excitation has significant autofluorescence background, which results in poor signal-to-noise ratio (SNR). Here we report TAT penetrating peptide-bioconjugated long persistent luminescence nanoparticles (LPLNP-TAT) for long-term tracking of adipose-derived stem cells (ASC) without constant external excitation. LPLNP-TAT exhibits near-infrared emitting, red light renewable capability, and superior in vivo imaging depth and SNR compared with conventional organic dye and quantum dots. Our findings show that LPLNP-TAT can successfully label ASC without impairing their proliferation and differentiation and can effectively track ASC in skin-regeneration and tumor-homing models. We believe that LPLNP-TAT represents a new generation of cell tracking probes and will have broad application in diagnosis and therapy. |
| Databáze: | OpenAIRE |
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