Ras transformation alters the composition of extracellular matrix proteoglycans in rat fibroblasts
| Autor: | Anna Molist, Manuel Romarı́s, Anna Bassols, Juan Villena, Ascensión Heredia |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Decorin
Biophysics Biochemistry Dermatan sulfate P21 RAS Protein Extracellular matrix chemistry.chemical_compound Sulfation Versicans Transforming Growth Factor beta Biglycan Animals Lectins C-Type Molecular Biology Cells Cultured Extracellular Matrix Proteins biology Temperature Cell Biology Fibroblasts Molecular biology Rats carbohydrates (lipids) Cell Transformation Neoplastic Genes ras Proteoglycan chemistry Chondroitin Sulfate Proteoglycans biology.protein Versican Proteoglycans |
| Zdroj: | Biochemical and biophysical research communications. 200(2) |
| ISSN: | 0006-291X |
| Popis: | ts371 KiMuSV-transformed rat kidney fibroblasts growing at the permissive temperature to allow the expression of a transforming p21 ras protein secrete a different pattern of chondroitin/dermatan sulfate proteoglycans than when growing at the non-permissive temperature. Transformed cells produce less versican and larger amounts of biglycan and decorin than their normal counterpart, and they do not secrete 35S-labeled PG-100. Western blot experiments showed the presence of PG-100 epitope, suggesting that the apparent effect of the oncoprotein is due to a reduced sulfation of the molecule. Transformed and non-transformed cells are similarly sensitive to the proteoglycan-induction effect of TGF-β. |
| Databáze: | OpenAIRE |
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