Dynamics of thymus-colonizing cells during human development
| Autor: | Frederic Jourquin, Rima Haddad, Isabelle André-Schmutz, Cecile Schiffer, Micael Yagello, Niclas Setterblad, Emmanuelle Six, Anne-Lise Delezoide, Bruno Canque, Marina Cavazzana-Calvo, Fabien Guimiot, Françoise Pflumio, Jean Claude Gluckman, Edmond Kahn |
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| Přispěvatelé: | Institut Universitaire d'Hématologie (IUH), Université Paris Diderot - Paris 7 (UPD7), Service de Biologie du Développement, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Robert Debré, Developpement Normal et Pathologique du Système Immunitaire, Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Service Commun d'Imagerie Cellulaire et Moléculaire, Université Paris Diderot - Paris 7 (UPD7)-Université Paris Diderot - Paris 7 (UPD7)-Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Laboratoire d'Imagerie Fonctionnelle (LIF), Université Pierre et Marie Curie - Paris 6 (UPMC)-IFR14-IFR49-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Cochin (UMR_S567 / UMR 8104), Saidi, Vanessa, Laboratoire d'Immunologie Cellulaire et Immunopathologie de l'Ecole Pratique des Hautes Etudes, Université Paris Diderot - Paris 7 ( UPD7 ) -Centre National de la Recherche Scientifique ( CNRS ), Assistance publique - Hôpitaux de Paris (AP-HP)-Hôpital Robert Debré, Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Institut Universitaire d'Hématologie ( IUH ), Université Paris Diderot - Paris 7 ( UPD7 ) -Université Paris Diderot - Paris 7 ( UPD7 ) -Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Laboratoire d'Imagerie Fonctionnelle ( LIF ), Université Pierre et Marie Curie - Paris 6 ( UPMC ) -IFR14-IFR49-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Institut Cochin ( UMR_S567 / UMR 8104 ), Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut Universitaire d'Hématologie (IUH), Université Paris Diderot - Paris 7 (UPD7)-Université Paris Diderot - Paris 7 (UPD7) |
| Rok vydání: | 2005 |
| Předmět: |
MESH : Bone Marrow
Antigens Differentiation T-Lymphocyte Pathology MESH: Hematopoietic Stem Cells Mice 0302 clinical medicine Bone Marrow Cell Movement Immunology and Allergy MESH : Cell Movement MESH: Animals [ SDV.IB ] Life Sciences [q-bio]/Bioengineering MESH: Antigens CD MESH: Cell Movement MESH : Organ Culture Techniques 0303 health sciences education.field_of_study B-Lymphocytes Cell Differentiation Phenotype MESH : Mice Transgenic Cell biology Haematopoiesis MESH : Phenotype MESH : Antigens Differentiation T-Lymphocyte medicine.anatomical_structure Infectious Diseases MESH: Bone Marrow [SDV.IB]Life Sciences [q-bio]/Bioengineering MESH : Cell Differentiation MESH: Cell Differentiation medicine.medical_specialty MESH: Mice Transgenic T cell Population Immunology Mice Transgenic Thymus Gland Biology MESH: Phenotype MESH : B-Lymphocytes 03 medical and health sciences Organ Culture Techniques Antigens CD MESH: B-Lymphocytes MESH : Mice medicine MESH : Antigens CD Animals Humans MESH : Thymus Gland Progenitor cell education MESH: Mice 030304 developmental biology [SDV.IB] Life Sciences [q-bio]/Bioengineering Fetus MESH: Humans MESH : Hematopoietic Stem Cells MESH : Humans MESH: Thymus Gland Hematopoietic Stem Cells MESH: Organ Culture Techniques MESH: Antigens Differentiation T-Lymphocyte Bone marrow MESH : Animals Ex vivo 030215 immunology |
| Zdroj: | Immunity Immunity, 2006, 24 (2), pp.217-30. ⟨10.1016/j.immuni.2006.01.008⟩ Immunity, Elsevier, 2006, 24 (2), pp.217-30. 〈10.1016/j.immuni.2006.01.008〉 Immunity, Elsevier, 2006, 24 (2), pp.217-30. ⟨10.1016/j.immuni.2006.01.008⟩ |
| ISSN: | 1074-7613 |
| DOI: | 10.1016/j.immuni.2006.01.008⟩ |
| Popis: | SummaryHere, we identify fetal bone marrow (BM)-derived CD34hiCD45RAhiCD7+ hematopoietic progenitors as thymus-colonizing cells. This population, virtually absent from the fetal liver (FL), emerges in the BM by development weeks 8–9, where it accumulates throughout the second trimester, to finally decline around birth. Based on phenotypic, molecular, and functional criteria, we demonstrate that CD34hiCD45RAhiCD7+ cells represent the direct precursors of the most immature CD34hiCD1a− fetal thymocytes that follow a similar dynamics pattern during fetal and early postnatal development. Histological analysis of fetal thymuses further reveals that early immigrants predominantly localize in the perivascular areas of the cortex, where they form a lymphostromal complex with thymic epithelial cells (TECs) driving their rapid specification toward the T lineage. Finally, using an ex vivo xenogeneic thymus-colonization assay, we show that BM-derived CD34hiCD45RAhiCD7+ progenitors are selectively recruited into the thymus parenchyma in the absence of exogenous cytokines, where they adopt a definitive T cell fate. |
| Databáze: | OpenAIRE |
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