Differential inhibition of rat and mouse microsome heme oxygenase by derivatives of imidazole and benzimidazole
| Autor: | Maaike Hum, Jason Z. Vlahakis, Brian E. McLaughlin, Walter A. Szarek, Kanji Nakatsu, Dragic Vukomanovic, Xianqi Kong |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Benzimidazole Physiology Stereochemistry Differential inhibition Gene Expression Regulation Enzymologic Mice 03 medical and health sciences chemistry.chemical_compound Physiology (medical) Animals Imidazole Enzyme Inhibitors Pharmacology Imidazoles Brain General Medicine Rats Heme oxygenase 030104 developmental biology chemistry Biochemistry Heme Oxygenase (Decyclizing) Microsome Benzimidazoles Female Spleen |
| Zdroj: | Canadian Journal of Physiology and Pharmacology. 95:1454-1461 |
| ISSN: | 1205-7541 0008-4212 |
| DOI: | 10.1139/cjpp-2017-0236 |
| Popis: | Metalloporphyrin heme oxygenase (HO) inhibitors have made an important contribution to elucidating the role of HO in physiological processes. Nevertheless, their off-target effects have drawn substantial criticism, which prompted us to develop non-porphyrin, azole-based inhibitors of HO. These second-generation HO inhibitors were evaluated using spleen and brain microsomes from rats as native sources of HO-1 and HO-2, respectively. Recently, the use of azole-based inhibitors of HO has been extended to other mammalian species and, as a consequence, it will be important to characterize the inhibitors in these species. The goal of this study was to compare the inhibitory profile of imidazole- and benzimidazole-based inhibitors of HO in a breast-cancer-implanted mouse to that of an untreated rat. For spleen and brain microsomes from both species, HO protein expression was determined by Western blotting and concentration–response curves for imidazole- and benzimidazole-derivative inhibition of HO activity were determined using a headspace gas-chromatographic assay. It was found that the effects on HO activity by imidazole and benzimidazole derivatives were different between the 2 species and were not explained by differences in HO expression. Thus, the HO inhibitory profile should be determined for azole derivatives before they are used in mammalian species other than rats. |
| Databáze: | OpenAIRE |
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