Toxicological evaluation of diesel emissions on A549 cells
| Autor: | Gen Sugiyama, Hiroki Okumura, Toru Nakajima, Naomi Tsukue, Tsuyoshi Ito |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Diesel exhaust
Serial dilution Cell Survival Nitrous Oxide Toxicology Diesel engine Polymerase Chain Reaction Cell Line chemistry.chemical_compound Humans Nitrogen dioxide Viability assay Food science Lung Oligonucleotide Array Sequence Analysis Vehicle Emissions A549 cell Dose-Response Relationship Drug Gene Expression Profiling General Medicine Particulates Dilution Culture Media chemistry Gene Expression Regulation Particulate Matter |
| Zdroj: | Toxicology in vitro : an international journal published in association with BIBRA. 24(2) |
| ISSN: | 1879-3177 |
| Popis: | To evaluate the health effects of diesel emissions (DE) using an in vitro experiment, A549 cells were exposed to emission from a diesel engine on an engine dynamo, using a culture-cell-exposure device. Three groups were set according to cell exposure to high concentrations of particulate matter (PM) and/or nitrogen dioxide (NO 2 ). The emissions of each group was dilution rate 1:100 and 1:10, and PM was 0.8 mg/m 3 and/or NO 2 was 80 ppm at dilution rate 1:10. After 1 h, exposed cells were analyzed for cell viability and gene expression. Fifty percent of cell viability in the high-PM/high-NO 2 exposure group occurred at a dilution rate of 1:14, based on the concentration of CO 2 . Heme oxygenase-1 mRNA expression significantly increased at 1:100 dilution of the high-PM/high-NO 2 group and 1:100 and 1:10 dilutions of the high-PM/low-NO 2 group, compared to background air. By DNA microarray, all gene expressions at a dilution rate of 1:10 in each group were observed to be higher than those at 1:100, and some cancer-related genes up-regulated. We concluded that screening methods for evaluating health effects could be established using this cell-exposure system because the effects of DE on A549 cells were shown by cell viability and gene expression. |
| Databáze: | OpenAIRE |
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