The use of suckling mice to isolate and grow Giardia from mammalian faecal specimens for genetic analysis
| Autor: | D. J. Merry, M. J. Albert, Ross H. Andrews, Graham Mayrhofer, Peter L. Ey, T. R. Grimmond |
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| Rok vydání: | 1992 |
| Předmět: |
Giardiasis
Genotype Genetic analysis Microbiology Feces Mice In vivo Cell Adhesion Parasite hosting Animals Humans Alleles Genetics biology Giardia Isolation (microbiology) biology.organism_classification In vitro Animals Suckling Isoenzymes Infectious Diseases Protozoa Animal Science and Zoology Parasitology |
| Zdroj: | Parasitology. 105 |
| ISSN: | 0031-1820 |
| Popis: | SUMMARYA simple technique is described for preparation ofGiardiacysts from faecal samples, the growth of trophozoites in suckling mice and the isolation of trophozoites for genetic analysis by allozyme electrophoresis. In total, 125 new isolates ofGiardiahave been collected from human and animal sources over a wide geographical area of South Australia and the Northern Territory of Australia. A number of long-established axenized isolates ofG. intestinalisbelonging to Groups I and II also adapted to grow in suckling mice. These findings indicate that suckling mice are permissive hosts for a variety of genetically dissimilar but morphologically similar organisms of theG. duodenalistype and that thisin vivotechnique may be less selective than isolation byin vitroculture. The use of suckling mice has revealed that infections can be composed of mixed genotypes and that isolation and purification techniques can be selective. Allozymic interpretation is essential to reveal the genetic complexity of such mixtures |
| Databáze: | OpenAIRE |
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