Poly(anhydride) nanoparticles containing cashew nut proteins can induce a strong Th1 and Treg immune response after oral administration
| Autor: | Priscila Valera Guerra, Juan M. Irache, Rafaela de Siqueira Ferraz-Carvalho, Inés Luis de Redín, Cláudia Brodskyn, Juliana de Souza Rebouças, Carlos Gamazo, Nereide S. Santos-Magalhães, Marcela Araújo Pereira |
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| Rok vydání: | 2018 |
| Předmět: |
CD4-Positive T-Lymphocytes
0301 basic medicine Allergy Administration Oral Pharmaceutical Science Context (language use) 02 engineering and technology Pharmacology T-Lymphocytes Regulatory Anhydrides Mice 03 medical and health sciences Immune system Oral administration Food allergy medicine Animals Nuts Mesenteric lymph nodes Anacardium Mice Inbred BALB C Chemistry digestive oral and skin physiology food and beverages FOXP3 General Medicine Allergens Th1 Cells 021001 nanoscience & nanotechnology medicine.disease 030104 developmental biology medicine.anatomical_structure Desensitization Immunologic Cytokines Nanoparticles Tree nut allergy Lymph Nodes Nut Hypersensitivity 0210 nano-technology Biotechnology |
| Zdroj: | European Journal of Pharmaceutics and Biopharmaceutics. 127:51-60 |
| ISSN: | 0939-6411 |
| DOI: | 10.1016/j.ejpb.2018.02.011 |
| Popis: | Cashew nut allergy is the second most commonly reported tree nut allergy. Traditional allergen immunotherapy presents several clinical drawbacks that can be reduced by using nanoparticles-based allergen-delivery systems, modulating the immune response towards a protective one. In this context, the goal of this work was to assess the potential of poly(anhydride) nanoparticles (NP) for cashew nut oral immunization. Cashew nut allergens-loaded nanoparticles (CNE-NP) were prepared by solvent displacement method. After nanoparticles characterization, oral immunomodulation ability was evaluated in BALB/c mice. Our results demonstrated that CNE-NP induced a higher Th1/Th2 ratio in comparison with animals immunized with free cashew nut proteins. Indeed, a decrease in splenic Th2 cytokines (IL-4, IL-5, and IL-13), and an enhancement of pro-Th1 (IL-12 and IFN-γ) and regulatory (IL-10) cytokines was observed. Furthermore, mice orally immunized with CNE-NP presented an increased expansion of CD4+ T regulatory cells, such as CD4+Foxp3+ and CD4+LAP+, in the mesenteric lymph nodes. In conclusion, oral immunization with a single dose of poly(anhydride) nanoparticles loaded with cashew nut proteins leaded to a pro-Th1 and Treg immune response. Furthermore, their immunomodulatory properties could be introduced as a new approach for management of cashew nut allergy. |
| Databáze: | OpenAIRE |
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