Assessment of the genetic diversity of Kenyan coconut germplasm using simple sequence repeat (SSR) markers
| Autor: | Najya Muhammed, Kimani N. Cyrus, Simon M. Githiri, Maurice E. Oyoo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Germplasm Kenya Genetic diversity Veterinary medicine biology Arecaceae biology.organism_classification Sequence repeat 01 natural sciences Applied Microbiology and Biotechnology 03 medical and health sciences Coconut palm (Cocos nucifera L. Arecaceae) germplasm simple sequence repeat (SSR) markers cluster analysis diversity 030104 developmental biology Cocos nucifera Genetic marker Botany Genetics Microsatellite Agronomy and Crop Science Molecular Biology 010606 plant biology & botany Biotechnology |
| Zdroj: | African Journal of Biotechnology; Vol 15, No 40 (2016); 2215-2223 |
| ISSN: | 1684-5315 |
| Popis: | Genetic diversity and relationship among 48 coconut individuals ( Cocos nucifera L.) collections from the Coastal lowland of Kenya were analyzed using 15 simple sequence repeat (SSR) primer pairs. Diversity parameters were calculated using Popgene Software version 1.31. The gene diversity values ranged from 0.0408 (CAC68) to 0.4861 (CAC23) with a mean value of 0.2839. The polymorphic information content (PIC) values ranged from 0.0400 to 0.3680 with a mean value of 0.2348. Marker CAC23 had the highest PIC and revealed highest gene diversity values in this study. Analysis of the molecular variation indicated that within individual variation was 98% while among materials, variation was low at 2% suggesting that molecular variation was not defined by region of production. Cluster analysis was constructed using DARwin program version 6.0. Forty eight (48) coconut individuals were clustered into three groups. Keywords: Coconut palm ( Cocos nucifera L., Arecaceae), germplasm, simple sequence repeat (SSR) markers, cluster analysis, diversity |
| Databáze: | OpenAIRE |
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