Hydroxamate siderophores of Histoplasma capsulatum
| Autor: | Anupama Tiwari, Kym F. Faull, Dexter H. Howard, Ramin Rafie |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Siderophore
Time Factors Iron Immunology Histoplasma Immunoblotting Siderophores Fungus Biology Hydroxamic Acids Microbiology Antibodies Mass Spectrometry Piperazines Hydrolysis Chelex 100 chemistry.chemical_compound Chelation Chromatography High Pressure Liquid Chelating Agents Dose-Response Relationship Drug Fungi imperfecti biology.organism_classification Infectious Diseases Biochemistry chemistry Polyclonal antibodies biology.protein Parasitology Fungal and Parasitic Infections |
| Zdroj: | Infection and immunity. 68(4) |
| ISSN: | 0019-9567 |
| Popis: | The zoopathogenic fungus Histoplasma capsulatum , like other eukaryotic aerobic microorganisms, requires iron for growth. Under conditions of low iron availability, the fungus secretes hydroxamates that function as siderophores (iron chelators). The experiments to be reported were designed to gather further information on the hydroxamate siderophores of H. capsulatum . The fungus was grown in a synthetic medium deferrated with the cationic exchange resin Chelex 100. Siderophores were detected after 4 days of incubation at 37°C in media containing 0.3 to 1.0 μM iron. The secretion was suppressed by 10 μM iron. The hydroxamates were purified by reverse-phase and size-exclusion chromatography. On the basis of ions observed during electrospray mass spectroscopy, five hydroxamate siderophores were tentatively identified: dimerum acid, acetyl dimerum acid, coprogen B, methyl coprogen B, and fusarinine (monomeric). A polyclonal antibody to dimerum acid was generated. This reagent cross-reacted with coprogen B and fusarinine. Thus, the antibody detects hydroxamates in all three families of siderophores excreted by H. capsulatum . |
| Databáze: | OpenAIRE |
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