Effect of the anticoagulant, storage time and temperature of blood samples on the concentrations of 27 multiplex assayed cytokines – Consequences for defining reference values in healthy humans
| Autor: | Svein Barene, Hilde Fure, Elin Storjord, Grete Bergseth, Judith K Ludviksen, Tom Eirik Mollnes, Linda Torrissen Hennø, Ole-Lars Brekke, Dorte Christiansen, Erik Waage Nielsen |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine medicine.medical_treatment Biochemistry Plasma 0302 clinical medicine Reference Values Immunology and Allergy Platelet Citrates Whole blood Aged 80 and over Immunoassay medicine.diagnostic_test VDP::Medical disciplines: 700::Clinical medical disciplines: 750 interleukin Chemistry Temperature VDP::Medisinske Fag: 700::Klinisk medisinske fag: 750 EDTA Interleukin Hematology Heparin Middle Aged Healthy Volunteers ethylenediamine tetraacetic acid Cytokine 030220 oncology & carcinogenesis Cytokines Female room temperature medicine.drug Adult Adolescent Immunology Complement RT Specimen Handling Sepsis Andrology Interferon-gamma Young Adult 03 medical and health sciences Lepirudin In vivo medicine Humans Molecular Biology Edetic Acid Aged Interleukin-8 Anticoagulant Anticoagulants IL medicine.disease 030104 developmental biology Interleukin-2 Biomarkers |
| Zdroj: | Cytokine |
| ISSN: | 1043-4666 |
| DOI: | 10.1016/j.cyto.2017.05.014 |
| Popis: | The article has been peer-reviewed, but does not include the publisher’s layout, page numbers and proof-correction. Citation for the published paper: Hennø, L. T., Storjord, E., Christiansen, D., Bergseth, G., Ludviksen, J. K., Fure, H., … Brekke, O. L. (2017). Effect of the anticoagulant, storage time and temperature of blood samples on the concentrations of 27 cytokines determined using a multiplex assay and the definition of reference values in a healthy human population. Cytokine, 97, 86-95. http://dx.doi.org/10.1016/j.cyto.2017.05.014 Cytokines are potentially useful biomarkers of sepsis and other inflammatory conditions. Many cytokines can be released by leukocytes and platelets after sampling. The sampling and processing techniques are consequently critically important to measure the in vivo levels. We therefore examined the effects of four different anticoagulants, EDTA, citrate, lepirudin, heparin compared to serum, on the levels of 27 different cytokines. The effects of storage temperature, freezing and thawing on the plasma cytokines were examined. Cytokines were analysed using a multiplex immunoassay. The cytokine levels in serum were significantly higher compared with plasma, consistent with release of cytokines in vitro during coagulation. In general, the lowest values for all cytokines were found in EDTA samples, stored on crushed ice, centrifuged within 4 h and thereafter stored at −80 °C. MCP-1 and MIP-1β levels were highest in heparin plasma and storage of blood for up to 4 h at room temperature significantly increased the interleukin (IL)-2, IL-6, IL-8, IFN-γ and GM-CSF levels in EDTA plasma, indicating post-sampling release. In contrast, the IP-10 levels were unaffected by sample storage at both temperatures. Our results indicate that the cytokines were more stable in plasma than in whole blood after sampling. Thus, cytokines should be analysed in EDTA plasma samples stored on ice and centrifuged within 4 h. Based on these data, the reference ranges of 27 cytokines in EDTA plasma in 162 healthy human donors were calculated. |
| Databáze: | OpenAIRE |
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