Restitution of single-cell defects in the mouse colon epithelium differs from that of cultured cells
| Autor: | Dorothee Günzel, Jörg-Dieter Schulzke, Alfred H. Gitter, Michael Fromm, Hanno Troeger, Jan F. Richter, Peter Florian |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Pathology medicine.medical_specialty Cytochalasin D Cell Survival Colon Physiology Cell Apoptosis Mice Inbred Strains In Vitro Techniques Biology Occludin Interferon-gamma Mice HT29 Cells Physiology (medical) Escherichia coli medicine Animals Humans Regeneration Intestinal Mucosa Microscopy Confocal Tumor Necrosis Factor-alpha Electric Conductivity Membrane Proteins Epithelial Cells Intestinal epithelium Actins Epithelium Restitution medicine.anatomical_structure Culture Media Conditioned Female Caco-2 Cells Wound healing |
| Zdroj: | American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 290:R1496-R1507 |
| ISSN: | 1522-1490 0363-6119 |
| DOI: | 10.1152/ajpregu.00470.2005 |
| Popis: | Integrity of colon epithelium is of crucial importance and, as small defects occur constantly, rapid repair (restitution) is essential. To investigate the mechanism of restitution, single-cell lesions were induced in mouse colonic surface epithelia by iontophoretic injection of Ca2+. Closure of the resulting defects was monitored using confocal laser scanning microscopy (CLSM), and functional sealing by electrophysiological techniques. Restitution was evaluated as the time constant τ of the exponential decrease in conductance of an induced leak and amounted to 0.28 min under control conditions. After 4 min, the leak was completely sealed. Repair was thus considerably faster than in previously investigated HT-29/B6 cells (τ = 5.73 min). As in cultured cells, cytochalasin D delayed restitution in native colon epithelia (τ = 0.69 min), indicating the involvement of actin in the healing process; however, no accumulation of actin surrounding the lesion was detected. Long-term incubation of epithelia with IFN-γ alone or in combination with TNF-α increased τ to 0.49 and 0.59 min, respectively. In contrast to cultured cells, TNF-α alone did not affect restitution. A brief ( |
| Databáze: | OpenAIRE |
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