Enhanced treatment of articular cartilage defect of the knee by intra-articular injection of Bcl-xL-engineered mesenchymal stem cells in rabbit model
| Autor: | Bin Hu, Qin Ma, Jun-Lin Ren, Tianqiu Mao, Yanpu Liu, Junrui Zhang |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Cartilage
Articular Cell Survival Cellular differentiation Blotting Western Green Fluorescent Proteins bcl-X Protein Biomedical Engineering Medicine (miscellaneous) Apoptosis Mesenchymal Stem Cell Transplantation Transfection Injections Intra-Articular Biomaterials Cell therapy Tissue engineering In Situ Nick-End Labeling medicine Animals Humans Viability assay Stem cell transplantation for articular cartilage repair Wound Healing Tissue Engineering business.industry Cartilage Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Genetic Therapy Flow Cytometry Culture Media Hindlimb Disease Models Animal medicine.anatomical_structure Cytoprotection Cancer research Rabbits Wound healing business Biomedical engineering |
| Zdroj: | Journal of Tissue Engineering and Regenerative Medicine. 4:105-114 |
| ISSN: | 1932-7005 1932-6254 |
| DOI: | 10.1002/term.212 |
| Popis: | Direct intra-articular injection of mesenchymal stem cells (MSCs) has been proposed as a potential cell therapy for cartilage defects. This cell therapy relies on the survival of the implanted MSCs. However, the arduous local environment may limit cell viability after implantation, which would restrict the cells' regenerative capacity. Thus, it is necessary to reinforce the implanted cells against the unfavourable microenvironment in order to improve the efficacy of cell therapy. We examined whether the transduction of an anti-apoptotic protein, Bcl-xL, into MSCs could prevent cell death and improve the implantation efficiency of MSCs in a rabbit model. Our current findings demonstrate that the group treated with Bcl-xL-engineered MSCs could improve cartilage healing both morphologically and histologically when compared with the controls. These results suggest that intra-articular injection of Bcl-xL-engineered MSCs is a potential non-invasive therapeutic method for effectively treating cartilage defects of the knee. |
| Databáze: | OpenAIRE |
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