Histopathological study on neuroapoptotic alterations induced by etomidate in rat hippocampus
Autor: | Mina Kamkar Asl, Fatemeh Alipour, Vahid Ebrahimi, Alireza Ebrahimzadeh-Bideskan, Mohammad Alipour, Amir Raoofi, Elnaz Khordad |
---|---|
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Male medicine.medical_specialty Histology medicine.medical_treatment Neurogenesis Intraperitoneal injection Hippocampus Stereology Apoptosis 03 medical and health sciences 0302 clinical medicine Etomidate Internal medicine medicine Animals Rats Wistar Saline Neurons TUNEL assay business.industry Dentate gyrus Cell Biology General Medicine 030104 developmental biology Endocrinology nervous system 030220 oncology & carcinogenesis business medicine.drug |
Zdroj: | Acta histochemica. 123(3) |
ISSN: | 1618-0372 |
Popis: | In human, there is substantial neurogenesis in the hippocampus that is implicated in memory formation and learning. These new-born neurons can be affected by neuropathological conditions. Anesthesia and surgical procedures are associated with postoperative cognitive changes particularly, impaired memory and learning. Therefore, the aim of this study was to evaluate the possible neurodegenerative effects of etomidate in rat hippocampus. Thirty male Wistar rats weighing 250 ± 30 g were randomly divided into 3 groups: 1) Etomidate group; four times 20 mg intraperitoneal injection with 1-h intervals, 2) Control group; the equal volume of normal saline, and 3) Normal group; without any intervention. 6 h after the last injection, the brains were removed and processed according to routine histological methods. TUNEL assay and toluidine blue staining were performed to evaluate neuro-histopathological changes in different regions of hippocampus. Our results showed that the number of TUNEL positive cells and dark neurons (DNs) in etomidate group were significantly higher in the CA1, CA2, CA3, and dentate gyrus (DG) of hippocampus compared with the control and normal groups (p 0.05). While, there was no significant difference between the various regions of hippocampus in control and normal groups. Our findings showed that etomidate can increase apoptotic cells and dark neurons induction in different regions of hippocampus mainly in DG. |
Databáze: | OpenAIRE |
Externí odkaz: |