Semi-quantitative duplex RT-PCR reveals the low occurrence of Porcine Pegivirus and Atypical Porcine Pestivirus in diagnostic samples from the United States
| Autor: | Stephanie Rossow, Fangzhou Chen, Douglas Marthaler, Todd P. Knutson, Eli Braun, Yin Jiang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Swine
Pegivirus Minnesota Short Communication Short Communications Biology Phylogenetics Porcine pegivirus Animals Clade Phylogeny atypical porcine pestivirus Swine Diseases General Veterinary General Immunology and Microbiology Coinfection Reverse Transcriptase Polymerase Chain Reaction Flaviviridae Pestivirus Infections General Medicine Flaviviridae Infections biology.organism_classification Virology United States phylogenetics Real-time polymerase chain reaction Atypical porcine pestivirus Duplex (building) Pestivirus Metagenomics Semi quantitative |
| Zdroj: | Transboundary and Emerging Diseases |
| ISSN: | 1865-1682 |
| Popis: | Summary Porcine Pegivirus (PPgV) and Atypical Porcine Pestivirus (APPV) are two recently identified porcine viruses. In this study, the identification of two viruses by metagenomic sequencing, and a duplex semi‐quantitative RT‐PCR was developed to detect these pathogens simultaneously. The PPgV strain Minnesota‐1/2016 had a 95.5%–96.3% nucleotide identity and clustered with the recently identified US PPgV strains, which is a distant clade from the German PPgV strains. The APPV strain Minnesota‐1/2016 shared an 87.3%–92.0% nucleotide identity with the other global APPV strains identity but only shared an 82.8%–83.0% nucleotide identity with clade II consisting of strain identified in China. Detection of both PPgV and APPV was 9.0% of the diagnostic cases. Co‐infection of PPgV and APPV was identified in 7.5% of the diagnostic cases. The occurrence and genetic characterization of PPgV and APPV further enhance our knowledge regarding these new pathogens in the United States. |
| Databáze: | OpenAIRE |
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