Formation of Lentivirus Particles by Mammalian Cells Infected with Recombinant Fowlpox Virus
| Autor: | Dennis Panicali, Lawrence Kenneth Cohen, Linda Gritz, Carolyn H. Fedor, Elizabeth M. O'neill, Sharon Jenkins |
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| Rok vydání: | 1991 |
| Předmět: |
viruses
Genetic Vectors Molecular Sequence Data Immunology DNA Recombinant Retroviridae Proteins Chick Embryo medicine.disease_cause Genes env Virus Cell Line law.invention law Virology medicine Animals Poxviridae Promoter Regions Genetic Cells Cultured Fowlpox virus Base Sequence biology virus diseases Simian immunodeficiency virus Avipoxvirus biology.organism_classification Genes gag Genes pol Microscopy Electron Infectious Diseases Chordopoxvirinae Capsid Recombinant DNA Simian Immunodeficiency Virus |
| Zdroj: | AIDS Research and Human Retroviruses. 7:991-998 |
| ISSN: | 1931-8405 0889-2229 |
| Popis: | Recombinant fowlpox viruses (FPV) containing the env or gag-pol genes of simian immunodeficiency virus from macaques (SIVmac) were constructed. The env, gag, and pol-encoded polypeptides were efficiently expressed and processed in avian cells productively infected with FPV as well as in mammalian cells, in which FPV infection is abortive. In addition, the recombinant FPV expressing the gag-pol genes directed the formation of defective, lentivirus-like particles which were released into the culture medium of infected cells. Coinfection of cells with the env and gag-pol recombinant viruses resulted in the generation of particles containing SIVmac envelope glycoprotein. The applications of this system to vaccine development are discussed. |
| Databáze: | OpenAIRE |
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