Upregulation of spinal ASIC1 by miR‐485 mediates enterodynia in adult offspring rats with prenatal maternal stress

Autor: Yan-Yan Wu, Cai-Lin Wang, Xue Xu, Ying Zhang, Yu-Cheng Xu, Rui-Xia Weng, Guang-Yin Xu, Yong-Chang Li, Ping-An Zhang
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Male
medicine.medical_specialty
Offspring
acid‐sensitive ion channel 1
prenatal maternal stress
In situ hybridization
Neurotransmission
Rats
Sprague-Dawley
03 medical and health sciences
0302 clinical medicine
Downregulation and upregulation
Dorsal root ganglion
Pregnancy
Physiology (medical)
Internal medicine
medicine
Animals
Pharmacology (medical)
Patch clamp
spinal dorsal horn
Pharmacology
business.industry
Antagonist
Age Factors
Original Articles
Abdominal Pain
Rats
Up-Regulation
Blot
Acid Sensing Ion Channels
Psychiatry and Mental health
MicroRNAs
030104 developmental biology
medicine.anatomical_structure
Endocrinology
enterodynia
miR‐485
Spinal Cord
Prenatal Exposure Delayed Effects
Original Article
Female
business
030217 neurology & neurosurgery
Stress
Psychological
Zdroj: CNS Neuroscience & Therapeutics
ISSN: 1755-5949
1755-5930
Popis: Aims Irritable bowel syndrome (IBS) is a common functional gastrointestinal disease characterized by abdominal pain. Our recent study has shown that the acid‐sensitive ion channel 1 (ASIC1) in dorsal root ganglion (DRG) is involved in stomachache of adult offspring rats subjected with prenatal maternal stress (PMS). MiR‐485 is predicted to target the expression of ASIC1. The aim of the present study was designed to determine whether miR‐485/ASIC1 signaling participates in enterodynia in the spinal dorsal horn of adult offspring rats with PMS. Methods Enterodynia was measured by colorectal distension (CRD). Western blotting, qPCR, and in situ hybridization were performed to detect the expression of ASICs and related miRNAs. Spinal synaptic transmission was also recorded by patch clamping. Results PMS offspring rats showed that spinal ASIC1 protein expression and synaptic transmission were significantly enhanced. Administration of ASICs antagonist amiloride suppressed the synaptic transmission and enterodynia. Besides, PMS induced a significant reduction in the expression of miR‐485. Upregulating the expression markedly attenuated enterodynia, reversed the increase in ASIC1 protein and synaptic transmission. Furthermore, ASIC1 and miR‐485 were co‐expressed in NeuN‐positive spinal dorsal horn neurons. Conclusions Overall, these data suggested that miR‐485 participated in enterodynia in PMS offspring, which is likely mediated by the enhanced ASIC1 activities.
The present study has shown that miR‐485 negatively regulates ASIC1 expression and synaptic transmission in the spinal dorsal horn, thus eventually contributing to enterodynia of PMS offspring. It tips us to prevent the adverse effects on offspring induced by environmental stressors during pregnancy.
Databáze: OpenAIRE
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