A fast perfusion system for single cell physiology optimized for microscopes with water immersion objectives
| Autor: | Frank Kirchhoff, Helmut Kettenmann, Carsten Ohlemeyer |
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| Rok vydání: | 1992 |
| Předmět: |
Measurement method
Microscope Physiology business.industry Chemistry System of measurement Cytological Techniques Clinical Biochemistry Pipette Cell Physiological Phenomena law.invention Perfusion Solutions Optics Microscopy Fluorescence law Water immersion Physiology (medical) Immersion Animals business Water immersion objective Micromanipulator |
| Zdroj: | Pfl�gers Archiv European Journal of Physiology. 420:573-577 |
| ISSN: | 1432-2013 0031-6768 |
| Popis: | A perfusion system was constructed which allows the fast application of different solutes underneath a water immersion objective. The perfusion system is mounted into the immersion objective by milling a slot into the frontal metal plate of the lens holder. It consists of a five-channel pipette fixed to the objective and solution reservoirs gated by computer controlled magnetic valves. Up to five different solutions can be applied to the specimen under study. The solution between objective and specimen is completely exchanged after 1-2 s as determined from fluorescence measurements. This arrangement is optimized for [Ca2+] measurements with a fluorescence measurement system in tissue slices, where upright microscopes are required. It offers the advantage of saving a micromanipulator for the perfusion pipette and facilitates a fast, reproducible and precise positioning of the perfusion system. |
| Databáze: | OpenAIRE |
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