Imaging microphysiological systems: a review
Autor: | Mark Jackman, Samantha Peel |
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Rok vydání: | 2021 |
Předmět: |
Automation
Laboratory 0301 basic medicine Microscopy Materials science Physiology Microfluidics 02 engineering and technology Cell Biology Microfluidic Analytical Techniques 021001 nanoscience & nanotechnology High-Throughput Screening Assays 03 medical and health sciences 030104 developmental biology Lab-On-A-Chip Devices Drug Discovery Fluorescence microscope Animals Humans Single-Cell Analysis 0210 nano-technology Cells Cultured High content imaging Biomedical engineering |
Zdroj: | American Journal of Physiology-Cell Physiology. 320:C669-C680 |
ISSN: | 1522-1563 0363-6143 |
Popis: | Microphysiological systems (MPS), often referred to as “organ-on-chips,” are microfluidic-based in vitro models that aim to recapitulate the dynamic chemical and mechanical microenvironment of living organs. MPS promise to bridge the gap between in vitro and in vivo models and ultimately improve the translation from preclinical animal studies to clinical trials. However, despite the explosion of interest in this area in recent years, and the obvious rewards for such models that could improve R&D efficiency and reduce drug attrition in the clinic, the pharmaceutical industry has been slow to fully adopt this technology. The ability to extract robust, quantitative information from MPS at scale is a key requirement if these models are to impact drug discovery and the subsequent drug development process. Microscopy imaging remains a core technology that enables the capture of information at the single-cell level and with subcellular resolution. Furthermore, such imaging techniques can be automated, increasing throughput and enabling compound screening. In this review, we discuss a range of imaging techniques that have been applied to MPS of varying focus, such as organoids and organ-chip-type models. We outline the opportunities these technologies can bring in terms of understanding mechanistic biology, but also how they could be used in higher-throughput screens, widening the scope of their impact in drug discovery. We discuss the associated challenges of imaging these complex models and the steps required to enable full exploitation. Finally, we discuss the requirements for MPS, if they are to be applied at a scale necessary to support drug discovery projects. |
Databáze: | OpenAIRE |
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