Trichostatin A induces apoptosis in oral squamous cell carcinoma cell lines independent of hyperacetylation of histones
| Autor: | Sung-Dae Cho, Kyung-Eun Lee, Boonsil Jang, Seung-Youp Lee, Lee-Han Kim, Ji-Ae Shin |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cell Survival MAP Kinase Signaling System viruses Apoptosis trichostatin A Hydroxamic Acids Apoptosis-inducing factor lcsh:RC254-282 Histones 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cell Line Tumor medicine Humans Radiology Nuclear Medicine and imaging Bim Cell Lineage Viability assay neoplasms Cell Proliferation biology Chemistry organic chemicals Acetylation General Medicine lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens oral squamous cell carcinoma Gene Expression Regulation Neoplastic stomatognathic diseases 030104 developmental biology Histone Trichostatin A Oncology Cell culture 030220 oncology & carcinogenesis biology.protein Cancer research Carcinoma Squamous Cell Trypan blue Mouth Neoplasms medicine.drug |
| Zdroj: | Journal of Cancer Research and Therapeutics, Vol 14, Iss 10, Pp 576-582 (2018) |
| ISSN: | 1998-4138 |
| Popis: | Aim of Study: To investigate the apoptotic event of trichostatin A (TSA) and its associated mechanism in oral squamous cell carcinoma (OSCC) lines. Materials and Methods: HSC-3 and Ca9.22 cell lines were evaluated using a trypan blue exclusion assay, histone isolation, soft agar assay, live/dead assay, 4%,6-diamidino-2-phenylindole staining, JC-1 mitochondrial membrane potential (MMP) assay, and Western blot analysis to demonstrate the anticancer activity of TSA. Results: TSA decreased OSCC cell viability and proliferation without affecting the histone acetylation. TSA-induced caspase-dependent or -independent apoptosis according to cell types, TSA enhanced the expression levels of Bim protein by dephosphorylating ERK1/2 pathway in HSC-3 cells. TSA also damaged MMP and increased cytosolic apoptosis-inducing factor (AIF) in Ca9.22 cells. Conclusion: The present study suggests that TSA may be a potential anticancer drug candidate for the treatment of OSCC through the induction of apoptosis. |
| Databáze: | OpenAIRE |
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