Berberine Modulates Gut Microbiota and Reduces Insulin Resistance via the TLR4 Signaling Pathway
| Autor: | Y. Zhang, Tieyun Zhao, Sheyu Li, Dan Liu, Liqiong Hou, Haoming Tian, Yunhui Liu |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Blood Glucose Lipopolysaccharides Male medicine.medical_specialty Berberine Endocrinology Diabetes and Metabolism Gut flora Diet High-Fat 03 medical and health sciences chemistry.chemical_compound Endocrinology Insulin resistance Internal medicine Internal Medicine medicine Animals Obesity Rats Wistar Triglycerides biology Triglyceride Tumor Necrosis Factor-alpha Fatty liver General Medicine Cholesterol LDL Coptis chinensis biology.organism_classification medicine.disease Gastrointestinal Microbiome Rats Fatty Liver Toll-Like Receptor 4 Insulin receptor Disease Models Animal 030104 developmental biology chemistry Liver TLR4 biology.protein lipids (amino acids peptides and proteins) Insulin Resistance Drugs Chinese Herbal Signal Transduction |
| Zdroj: | Experimental and clinical endocrinologydiabetes : official journal, German Society of Endocrinology [and] German Diabetes Association. 126(8) |
| ISSN: | 1439-3646 |
| Popis: | Berberine, a natural compound extracted from several Chinese herbs including Coptis chinensis, has been shown to have anti-obesity effects and prevents insulin resistance in high-fat diet (HFD)-fed obese rats by modulating the gut microbiota; however, the molecular mechanisms underlying these activities remain unknown. We investigated the effects of berberine on obesity and insulin resistance by examining the lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4)/tumor necrosis factor (TNF)-α signaling pathway in livers of HFD-fed obese rats. Our results showed that 8-week berberine (200 mg/kg) treatment significantly reduced fasting blood glucose, triglyceride, low-density lipoprotein-cholesterol and insulin resistance in HFD-fed obese rats. However, berberine had no significant effects on body weight, visceral fat mass or the visceral fat to body weight ratio. Berberine also attenuated HFD-induced hepatic steatosis. A prolonged HFD altered the gut microbiota composition by reducing protective bacteria like Bifidobacterium and increasing gram negative bacteria like Escherichia coli, which resulted in increased LPS release into plasma. Berberine reversed these effects and inhibited LPS-induced TLR4/TNF-α activation, resulting in increased insulin receptor and insulin receptor substrate-1 expression in the liver. These findings suggested that berberine may reduce insulin resistance, at least in part by modulating the gut microbiota along with inhibiting LPS/TLR4/TNF-α signaling in the liver. |
| Databáze: | OpenAIRE |
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