Construction of an opal suppressor by oligonucleotide-directed mutagenesis of a Saccharomyces cerevisiae tRNA(Trp) gene
| Autor: | A. L. Atkin, K. L. Roy, J. B. Bell |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1990 |
| Předmět: |
Base Sequence
Transcription Genetic Genes Fungal Molecular Sequence Data Restriction Mapping Cell Biology RNA Transfer Amino Acid-Specific RNA Transfer Trp Suppression Genetic Mutation Nucleic Acid Conformation Cloning Molecular DNA Fungal Oligonucleotide Probes Molecular Biology Alleles Research Article Plasmids |
| Popis: | In vitro mutagenesis was used to create putative opal suppressor alleles of a tRNA(Trp) gene of Saccharomyces cerevisiae. The construct with the requisite anticodon change did not result in an active suppressor, whereas when a second change was introduced into the portion of the gene encoding the intron, an active and specific opal suppressor was produced. We propose that the secondary structure of transcripts from the first mutant may prevent efficient pre-tRNA processing, whereas normal processing occurs with the double mutant. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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