A multiplex RT-PCR method to detect papaya meleira virus complex in adult pre-flowering plants
| Autor: | Tathiana F. Sá-Antunes, Alexandre Martins Costa Santos, Patricia Machado Bueno Fernandes, Scarlett A. Oliveira, Marlonni Maurastoni, José A. Ventura |
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| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Papaya meleira virus medicine.medical_specialty Serial dilution Carica Reverse Transcriptase Polymerase Chain Reaction 030306 microbiology General Medicine Biology Virology Virus 03 medical and health sciences Titer Plasmid Medical microbiology Real-time polymerase chain reaction Molecular Diagnostic Techniques medicine Multiplex Multiplex Polymerase Chain Reaction Plant Diseases Totiviridae 030304 developmental biology |
| Zdroj: | Archives of Virology. 165:1211-1214 |
| ISSN: | 1432-8798 0304-8608 |
| DOI: | 10.1007/s00705-020-04588-5 |
| Popis: | Papaya sticky disease (PSD), which can destroy orchards, was first attributed to papaya meleira virus (PMeV). However, the discovery of papaya meleira virus 2 (PMeV2) associated with PSD plants impose the need to detect this viral complex. We developed a multiplex RT-PCR (mPCR) technique capable of detecting two viruses in a single assay from pre-flowering plant samples, which is a useful tool for early diagnosis of PSD. We also determined the limit of detection (LOD) using asymmetric plasmid dilutions of both PMeV and PMeV2, which revealed that a higher titer of one virus prevents detection of the other. Thus, this technique is an alternative method for detecting PMeV and PMeV2 in a single reaction. |
| Databáze: | OpenAIRE |
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