Immunogenicity of two oat varieties, in relation to their safety for celiac patients
| Autor: | Gaetano Iaquinto, Carmen Gianfrani, Mariantonia Maglio, Nicola Giardullo, Rosita Stefanile, Giuseppe Mazzarella, Laura Gazza, Alessandra Camarca, Maria Vittoria Barone, B. Colicchio, Erasmo Miele, Norberto Pogna, Salvatore Auricchio, Merlin Nanayakkara, Pasquale Santoro, Riccardo Troncone, Francesco Maurano |
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| Přispěvatelé: | Maglio, M, Mazzarella, G, Barone, MARIA VITTORIA, Gianfrani, C, Pogna, N, Gazza, L, Stefanile, R, Camarca, A, Colicchio, B, Nanayakkara, M, Miele, Erasmo, Iaquinto, G, Giardullo, N, Maurano, F, Santoro, P, Troncone, Riccardo, Auricchio, S. |
| Rok vydání: | 2011 |
| Předmět: |
Adult
medicine.medical_specialty food.ingredient Adolescent Avena CD3 Complex Enterocyte Biopsy T cell CD3 Biology Lymphocyte Activation Gliadin Interferon-gamma Young Adult food T-Lymphocyte Subsets Interferon Internal medicine Electric Impedance medicine Humans IL-2 receptor Intestinal Mucosa Phosphorylation Child Extracellular Signal-Regulated MAP Kinases Cell Proliferation Interleukin-15 Lamina propria Interleukin-2 Receptor alpha Subunit Gastroenterology food and beverages Middle Aged Celiac Disease Enterocytes medicine.anatomical_structure Endocrinology Interleukin 15 Child Preschool biology.protein Caco-2 Cells medicine.drug |
| Zdroj: | Scandinavian Journal of Gastroenterology. 46:1194-1205 |
| ISSN: | 1502-7708 0036-5521 |
| DOI: | 10.3109/00365521.2011.603159 |
| Popis: | Objective. Most of the recent studies suggest that oats are well tolerated by celiac disease (CD) patients. However, it is still possible that different oat cultivars may display different biological properties relevant for CD pathogenesis. We aimed to investigate biological and immunological properties of two oat varieties, Avena genziana and Avena potenza, in relation to their safety for CD patients. Material and Methods. Phosphorylation of extracellular signal-regulated kinase (ERK) and transepithelial electrical resistance (TEER) were evaluated in CaCo-2 cells treated with peptic–tryptic (PT) digests from the two oats and from gliadin (PTG). With the same PT-digests, duodenal biopsies from 22 CD patients were treated in vitro for 24 h and density of CD25 + cells in lamina propria and of intraepithelial CD3 + T cells was measured, as well as crypt cell proliferation and epithelial expression of interleukin 15. Finally, interferon g (IFN-g) production was measured as evidence of gliadin-specific T-cell activation by PT-digests. Results. In contrast to PTG, oats PT-digests were not able to induce significant increase in ERK phosphorylation and decrease in TEER in CaCo-2 cells. In the organ culture system, oats PTdigests, unlike PTG, did not induce significant increase in crypt enterocyte proliferation, increase in interleukin 15 expression or in lamina propria CD25 + cells. Nevertheless Avena potenza increased intraepithelial T-cell density, while Avena genzianainduced IFN-g production in 3/8 CD intestinal T cell lines. Conclusions. Our data show that Avena genziana and Avena potenza do not display in vitro activities related to CD pathogenesis. Some T-cell reactivity could be below the threshold for clinical relevance. |
| Databáze: | OpenAIRE |
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