Establishment of a Primary Effusion Lymphoma Cell Line (RM-P1) and In Vivo Growth System Using SCID Mice
| Autor: | Akitoshi Nagasaki, Tetsuharu Shinjyo, Hiroshi Uezato, Jun-ichi Miyagi, Naoki Kakazu, Yuetsu Tanaka, Nobuyuki Takasu, Masato Masuda |
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| Rok vydání: | 2002 |
| Předmět: |
Male
Herpesvirus 4 Human Pathology medicine.medical_specialty Lymphoma CD58 Mice SCID Biology medicine.disease_cause Mice Immunophenotyping hemic and lymphatic diseases Tumor Cells Cultured medicine Animals Humans Severe combined immunodeficiency Cell adhesion molecule Hematology Middle Aged Cell sorting Intercellular adhesion molecule medicine.disease Epstein–Barr virus Molecular biology Pleural Effusion Malignant Karyotyping Herpesvirus 8 Human Primary effusion lymphoma Cell Adhesion Molecules Cell Division Neoplasm Transplantation |
| Zdroj: | International Journal of Hematology. 76:165-172 |
| ISSN: | 1865-3774 0925-5710 |
| Popis: | Primary effusion lymphoma (PEL) is recognized as a unique lymphoma entity, which occurs exclusively in body cavities as a serous lymphomatous effusion without tumor formation or organ infiltration. We established a cell line of B-cell origin from a pericardial effusion of a 63-year-old Japanese PEL patient who did not have human immunodeficiency virus infection. This PEL cell line had human herpesvirus-8 (HHV-8) and Epstein-Barr virus (EBV) infection. We named this cell line RM-P1. This cell line showed complex chromosomal abnormalities that could not be identified by G-banding. However, spectral karyotyping analysis determined the origin and organization of all unidentified chromosomal abnormalities. When inoculated into the peritoneal cavity of 8 severe combined immunodeficiency (SCID) mice depleted of natural killer cells, RM-P1 cells induced solid tumor with ascites in all animals tested. These tumor and ascitic cells had the same immunogenotypic features as those of the original RM-P1. These 2 types of cells were positive for both HHV-8 and EBV as demonstrated using polymerase chain reaction. Fluorescence-activated cell sorting analyses showed that neither tumors nor ascitic cells grown in SCID mice expressed leukocyte function-associated antigen (LFA)-1alpha (CD11a), LFA-1lbeta (CD18), LFA-2 (CD2), LFA-3 (CD58), intercellular adhesion molecule (ICAM)-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), or leukocyte endothelial adhesion molecule (LECAM)-1 (CD62L), suggesting that these cytoadhesion molecules are not involved in tumor formation of RM-P1 cells in vivo. The establishment of the RM-P1 cell line and the animal model of PEL may provide insights for understanding the relationship between these viruses and PEL and for understand the mechanism for PEL. |
| Databáze: | OpenAIRE |
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