Gene expression levels of the insulin-like growth factor family in patients with AMD before and after ranibizumab intravitreal injections
| Autor: | Urszula Mazurek, Adam Kabiesz, Małgorzata Kimsa-Furdzik, Małgorzata Nita, Katarzyna Michalska-Małecka, Barbara Strzalka-Mrozik |
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| Rok vydání: | 2017 |
| Předmět: |
Male
genetic structures medicine.medical_treatment mRNA Visual Acuity Gene Expression Angiogenesis Inhibitors Peripheral blood mononuclear cell Andrology 03 medical and health sciences Insulin-like growth factor Macular Degeneration 0302 clinical medicine Insulin-Like Growth Factor II Ranibizumab Gene expression Medicine Humans 030212 general & internal medicine RNA Messenger age-related macular degeneration Aged Original Research Aged 80 and over Messenger RNA business.industry Growth factor Insulin-like growth factor 2 receptor General Medicine Macular degeneration Middle Aged medicine.disease Clinical Interventions in Aging 030220 oncology & carcinogenesis Intravitreal Injections Leukocytes Mononuclear Female Geriatrics and Gerontology business IGF family medicine.drug |
| Zdroj: | Clinical Interventions in Aging |
| ISSN: | 1178-1998 |
| Popis: | Barbara Strzalka-Mrozik,1 Malgorzata Kimsa-Furdzik,2 Adam Kabiesz,3 Katarzyna Michalska-Malecka,3,4 Malgorzata Nita,5 Urszula Mazurek1 1Department of Molecular Biology, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Katowice, Poland; 2Department of Biochemistry, School of Medicine in Katowice, Medical University of Silesia, Katowice, Poland; 3University Center for Ophthalmology and Oncology, Independent Public Clinical Hospital, Medical University of Silesia, Katowice, Poland; 4Department of Ophthalmology, School of Medicine in Katowice, Medical University of Silesia, Katowice, Poland; 5Domestic and Specialized Medicine Centre “Dilmed”, Katowice, Poland Purpose: The present study focused on the assessment of the mRNA levels of the insulin-like growth factor (IGF) family in patients with the exudative form of age-related macular degeneration (AMD) before and after ranibizumab intravitreal injections.Patients and methods: An analysis of the expression profile of the IGF family of genes in patients with AMD was carried out using the oligonucleotide microarray and quantitative reverse transcriptase polymerase chain reaction (RT-qPCR) methods.Results: In the peripheral blood mononuclear cells (PBMCs) obtained from AMD group receiving ranibizumab compared to the peripheral blood mononuclear cells from AMD group before ranibizumab treatment using oligonucleotide microarray technique, six statistically significant differentially expressed transcripts related to the IGF family were detected (unpaired t-test, p1.5). Moreover, analysis using the real-time RT-qPCR technique revealed statistically significant differences in the IGF2 and IGF2R mRNA levels (Mann–Whitney U test, p |
| Databáze: | OpenAIRE |
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