The biological effects of rainbow trout (Oncorhynchus mykiss) recombinant interleukin-8
| Autor: | Pin Nie, Yang Yang Zhang, Christopher J. Secombes, Jun Zou, Nor Omaima Harun |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Neutrophils
medicine.medical_treatment Immunology Cell Count chemical and pharmacologic phenomena Recombinant Interleukin Peritoneal cavity Cell Movement parasitic diseases Escherichia coli Leukocytes medicine Animals Interleukin 8 Respiratory Burst Dose-Response Relationship Drug biology Interleukin-8 hemic and immune systems Biological activity biology.organism_classification Molecular biology Recombinant Proteins biological factors Respiratory burst Trout Cytokine medicine.anatomical_structure Oncorhynchus mykiss Rainbow trout Developmental Biology |
| Zdroj: | Developmental & Comparative Immunology. 32:673-681 |
| ISSN: | 0145-305X |
| Popis: | In this report, recombinant interieukin-8 (rIL-8) was produced and its activity tested for the first time in fish. The rainbow trout rIL-8 was produced in Escherichia coli and purified using a 6xHis tag at the N-terminus. The rIL-8 induced a dose-dependent migration of head kidney leukocytes at concentrations from 0.1 to 10 ng/ml, with a peak response at 1 ng/ml. Trout rIL-8 also had a significant effect on superoxide production by head kidney cells, with maximal, activity at 0.1 and 1 ng/ml. When injected intraperitoneally into trout, rIL-8 had a clear effect on total leukocyte number in the peritoneal cavity, with increasing doses (up to 5 mu g) eliciting more cells. Of three leukocyte types distinguished, neutrophils were the dominant cell type, especially at higher rIL-8 concentrations. In contrast, the proportion of macrophages and lymphocytes decreased with rIL-8 administration, suggesting that they were not attracted at the same rate as neutrophils. (C) 2007 Elsevier Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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