Altered hepatic retinol and CYP26 levels in adult European common frogs (Rana temporaria) exposed to p,p′-DDE
| Autor: | Bjørn Munro Jenssen, Anne S. Mortensen, Augustine Arukwe, Àngels Leiva-Presa |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Vitamin Insecticides medicine.medical_specialty medicine.drug_class Dichlorodiphenyl Dichloroethylene Rana temporaria Enzyme-Linked Immunosorbent Assay Aquatic Science Biology Oceanography Gene Expression Regulation Enzymologic Rana chemistry.chemical_compound Cytochrome P-450 Enzyme System Internal medicine medicine Animals Retinoid Vitamin A Chromatography High Pressure Liquid DNA Primers Sexual differentiation Dose-Response Relationship Drug Reverse Transcriptase Polymerase Chain Reaction Retinol Environmental Exposure General Medicine Retinoic Acid 4-Hydroxylase Pesticide Pollution Endocrinology Liver chemistry Environmental Pollutants Drug metabolism Homeostasis |
| Zdroj: | Marine Environmental Research. 62:S10-S15 |
| ISSN: | 0141-1136 |
| DOI: | 10.1016/j.marenvres.2006.04.013 |
| Popis: | The effects of environmental contamination on amphibians are of particular concern because there are reports of declining numbers of species and individuals in most parts of the world during the last 50 years. During the last decade there has been increased focus on the role of persistent organic pollutants as retinoid (vitamin A) disrupters, and their effects on development, growth and sexual differentiation. To study the effects of p,p'-DDE, one of the most persistent metabolites of the pesticide DDT, on retinol homeostasis, we subcutaneously exposed adult male European common frogs (Rana temporaria) to different doses of p,p'-DDE (0, 0.01, 0.1, 1 and 10 mg/kg body mass) and studied the effect of a short term exposure (14 days) on hepatic retinoid levels and CYP26 gene and protein expression. Hepatic retinol concentrations, CYP26 gene and protein levels were analysed using HPLC, quantitative RT-PCR and indirect ELISA, respectively. Our results showed a significant p,p'-DDE dose-specific increase in the hepatic retinol concentration. CYP26 gene and protein expression were reduced in an apparent p,p'-DDE dose-specific manner. The results suggest that p,p'-DDE may interfere with the hepatic metabolism of retinol in adult frogs by decreasing CYP26 expression patterns. |
| Databáze: | OpenAIRE |
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